Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD, this assay utilized an Ade2 reporter gene which the authors claim is more stringent than a LacZ/His3 reporter assay.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD, this screen utilized the activation of both a LacZ and His3 reporter gene.