FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
14-3-3ε, ncd
FlyBase ID
FBig0000115712
Interaction Network
Interactions Browser links
14-3-3ε network
ncd network
Reported Interactions
FBrf0236847-1.PD.MS
Reference
Beaven et al., 2017
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

GST tag

ncd 

Ncd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type ovary extract.

(Beaven et al., 2017)
FBrf0236847-2.PD.WB
Reference
Beaven et al., 2017
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

GST tag

ncd 

Ncd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type ovary extract.

(Beaven et al., 2017)
FBrf0236847-3.PD.WB
Reference
Beaven et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

GST tag

ncd 

Ncd

prey

GFP tag

ncd 
ncd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ncd 
interaction domain
mutation decreasing interaction
aa 96aa 94

S96A or S96D

S94D

ncd 
interaction domain
mutation disrupting interaction
aa 79,96,114

S79A,S96A,S114A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct.

(Beaven et al., 2017)
FBrf0236847-4.PD.MW
Reference
Beaven et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

14-3-3ε

bait

MBP tag

ncd 

Ncd

prey
ncd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ncd 
interaction domain
mutation decreasing interaction
aa 96

S96A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction requires S96 phosphorylated form of ncd.

(Beaven et al., 2017)

tion in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Beaven et al., 2017)

Phosphorylation at ncd S94 via treatment with aurB in addition to S96 phosphorylation disrupts the ncd-14-3-3ε interaction.

(Beaven et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)