FB2025_05 , released December 11, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
His4, l(3)mbt
FlyBase ID
FBig0000115717
Interaction Network
Interactions Browser links
His4 network
l(3)mbt network
Reported Interactions
FBrf0207121-3.PD.WB
Reference
Scharf et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His4 

H4

bait
His4 
l(3)mbt 

dl(3)MBT

prey

GST or FLAG tag

l(3)mbt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
l(3)mbt 
MBT1-3 domain
sufficient binding region
unspecified
His4 
interaction domain
sufficient binding region
aa 16-25
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide chemically synthesized; prey produced as a recombinant fusion protein in bacterial or baculovirus and insect cell systems.

(Scharf et al., 2009)

l(3)mbt binds preferentially to His4 mono- or dimethylated at K20.

(Scharf et al., 2009)
External Crossreferences and Linkouts ( 0 )
References (1)