FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
pll, D
FlyBase ID
FBig0000115741
Interaction Network
Interactions Browser links
pll network
D network
Reported Interactions
FBrf0211847-1.PD.A
Reference
Mutsuddi et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
D 

Dichaete

bait

GST tag

pll 

Pelle

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Mutsuddi et al., 2010)
FBrf0211847-2.PD.A
Reference
Mutsuddi et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pll 

Pelle

bait

GST tag

pll 
pll 
D 

Dichaete

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pll 
N-terminal domain
sufficient binding region
aa 1-209
pll 
C-terminal domain
sufficient binding region
aa 209-501
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Mutsuddi et al., 2010)

The N-terminal portion of pll binds to D more strongly than the C-terminal portion.

(Mutsuddi et al., 2010)
FBrf0211847-3.Y2H
Reference
Mutsuddi et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
D 

Dichaete

bait

fused to lexA DNA-binding domain

pll 

Pelle

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Mutsuddi et al., 2010)

Two-hybrid system: yeast lexA-BD/B42-AD

(Mutsuddi et al., 2010)
FBrf0211847-4.Y2H
Reference
Mutsuddi et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pll 

Pelle

bait

fused to lexA DNA-binding domain

pll 
pll 
D 

Dichaete

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pll 
N-terminal domain
sufficient binding region
aa 1-209
pll 
C-terminal domain
sufficient binding region
aa 209-501
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Mutsuddi et al., 2010)

Two-hybrid system: yeast lexA-BD/B42-AD, The N-terminal portion of pll binds to D more strongly than the C-terminal portion.

(Mutsuddi et al., 2010)
FBrf0211847-6.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pll 

Pelle

enzyme

His tag

pll 

Pelle

enzyme

His tag

D 

Dichaete

enzyme target

His tag, P[32] label

D 

Dichaete

enzyme target

His tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)