FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mical, Abl
FlyBase ID
FBig0000115824
Interaction Network
Interactions Browser links
Mical network
Abl network
Reported Interactions
FBrf0236717-1.Y2H
Reference
Yoon et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mical 

Mical

bait

fused to LexA DNA-binding domain

Mical 
Mical 
Mical 
Abl 

Abl

prey

fused to B42 activation domain

Abl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mical 
PxxP motif region
sufficient binding region
aa 2171-2550

coordinates relative to Mical-PE

Mical 
PxxP motif region
necessary binding region
unspecified

region described as "to the right of aa 2398", coordinates relative to Mical-PE

Mical 
PxxP motif region
mutation decreasing interaction
aa 2529-2533

PPLPL to KKLKK, coordinates relative to Mical-PE

Abl 
SH3 domain
sufficient binding region
aa 183-254

coordinates relative to Abl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Yoon et al., 2017)

Two-hybrid system: yeast LexA-BD/B42-AD

(Yoon et al., 2017)
FBrf0236717-2.PD.WB
Reference
Yoon et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

bait

GST tag

Abl 
Mical 

Mical

prey

His tag

Mical 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mical 
PxxP motif region
sufficient binding region
aa 2171-2550

coordinates relative to Mical-PE

Abl 
SH3 domain
sufficient binding region
aa 183-254

coordinates relative to Abl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yoon et al., 2017)
FBrf0236717-3.coIP.WB
Reference
Yoon et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abl 

Abl

bait

HA tag

Mical 

Mical

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Yoon et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)