FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000115875
Interaction Network
Interactions Browser links
Dyrk2 network
Reported Interactions
FBrf0192426-17.ENZ.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dDyrk2

putative self

FLAG tag

dDyrk2

putative self

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DH box
mutation decreasing interaction
unspecified

deletion of DH-box

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; enzyme produced as a recombinant fusion protein in baculovirus and Sf9 cell system.

FBrf0214988-1.ENZ.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dDYRK2

self

FLAG tag

dDYRK2

self

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
NAPA-1 domain
necessary binding region
aa 140-163

coordinates relative to Dyrk2-PA

interaction domain
sufficient binding region
aa 121-722aa 1-508

coordinates relative to Dyrk2-PA

coordinates relative to Dyrk2-PA

kinase domain residue 227
mutation disrupting interaction
aa 227

K227M, coordinates relative to Dyrk2-PA

NAPA-2 domain residue 192
mutation disrupting interaction
aa 192

D192V, coordinates relative to Dyrk2-PA

NAPA-2 domain residue 193
mutation disrupting interaction
aa 193

H193R, coordinates relative to Dyrk2-PA

NAPA-1 domain residue 140
mutation decreasing interaction
aa 140

L140E, coordinates relative to Dyrk2-PA

NAPA-1 domain residue 144
mutation decreasing interaction
aa 144

E144L, coordinates relative to Dyrk2-PA

NAPA-1 domain residue 147
mutation decreasing interaction
aa 147

E147L, coordinates relative to Dyrk2-PA

NAPA-1 domain residue 149
mutation decreasing interaction
aa 149

K149I, coordinates relative to Dyrk2-PA

NAPA-1 domain residue 158
mutation decreasing interaction
aa 158

G158E, coordinates relative to Dyrk2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; enzyme produced as a recombinant fusion protein in baculovirus and Sf9 cell system.

Study of tyrosine autophosphorylation by anti-phospho-Tyr western blot. The N-terminal 197 residues of Dyrk2 are important for tyrosine autophosphorylation and can complement autophosphorylation deficient N-terminal deletions when added in trans.

External Crossreferences and Linkouts ( 0 )
References (2)