FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
MtnB, Prx2
FlyBase ID
FBig0000115895
Interaction Network
Interactions Browser links
MtnB network
Prx2 network
Reported Interactions
FBrf0208070-1.Y2H
Reference
Tio et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MtnB 

MtnB

bait

fused to GAL4 DNA-binding domain

Prx2 

Jafrac1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult cDNA expression library, IL4002AH Clontech).

(Tio et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Tio et al., 2009)
FBrf0208070-3.PD.WB
Reference
Tio et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MtnB 

MtnB

bait

GST tag

Prx2 

Prx1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Tio et al., 2009)

Interaction observed when MtnB is produced without metal or in the presence of Zn but not in the presence of Cu. The addition of Cu to preformed Zn-MtnB complexes disrupts the MtnB-Jafrac1 interaction.

(Tio et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)