FlyBase Gene Report: Dmel\Prx2

General Information

Symbol

Dmel\Prx2

Species

D. melanogaster

Name

Peroxiredoxin 2

Annotation Symbol

CG1633

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040309

Gene Model Status

Current

Stock Availability

11 publicly available

Enzyme Name (EC)

thioredoxin-dependent peroxiredoxin (1.11.1.24)

Gene Summary

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity). (UniProt, Q9V3P0)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Jafrac1, thioredoxin peroxidase 1, Jafrac, DPx4783, DPx-4783

Key Links

Genomic Location

Cytogenetic map

11E11-11E11

Sequence location

X:13,329,824..13,331,994 [+]

Recombination map

1-42

RefSeq locus

NC_004354 REGION:13329824..13331994

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables peroxiredoxin activity

inferred from direct assay

(McGinnis et al., 2021)

enables thioredoxin-dependent peroxiredoxin activity

inferred from direct assay

(Bauer et al., 2002, Radyuk et al., 2001)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables antioxidant activity

inferred from electronic annotation with InterPro:IPR000866

(InterPro Project Members, 2004-)

enables oxidoreductase activity

inferred from electronic annotation with InterPro:IPR000866

(InterPro Project Members, 2004-)

enables peroxiredoxin activity

inferred from electronic annotation with InterPro:IPR019479

(InterPro Project Members, 2004-)

enables thioredoxin peroxidase activity

inferred from biological aspect of ancestor with PANTHER:PTN000073874

(GO Reference Genome Project, 2011-)

enables thioredoxin-dependent peroxiredoxin activity

non-traceable author statement

(Rodriguez et al., 2000)

Biological Process (10 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in cell adhesion

inferred from mutant phenotype

(Degennaro et al., 2011)

involved_in cell redox homeostasis

inferred from direct assay

(Bauer et al., 2002)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Biteau et al., 2010)

involved_in germ cell development

inferred from mutant phenotype

(Degennaro et al., 2011)

involved_in germ cell migration

inferred from mutant phenotype

(Degennaro et al., 2011)

involved_in hydrogen peroxide catabolic process

inferred from direct assay

(Radyuk et al., 2001)

involved_in response to oxidative stress

inferred from mutant phenotype

(Degennaro et al., 2011)

involved_in response to starvation

inferred from mutant phenotype

(Biteau et al., 2010)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in cell redox homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000073874

(GO Reference Genome Project, 2011-)

involved_in hydrogen peroxide catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000073874

(GO Reference Genome Project, 2011-)

involved_in leukocyte activation

inferred from biological aspect of ancestor with PANTHER:PTN000743557

(GO Reference Genome Project, 2011-)

involved_in removal of superoxide radicals

inferred from biological aspect of ancestor with PANTHER:PTN000743557

(GO Reference Genome Project, 2011-)

involved_in response to oxidative stress

inferred from biological aspect of ancestor with PANTHER:PTN000073874

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Radyuk et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN000073874

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

PEROXIREDOXINS

Protein Family (UniProt)

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily. (Q9V3P0)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

thioredoxin-dependent peroxiredoxin activity

a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]- disulfide + H2O (1.11.1.24)

RHEA 62620: peroxiredoxin activity
RHEA 10008: thioredoxin peroxidase activity
RHEA 63528:

Summaries

Gene Group (FlyBase)

PEROXIREDOXINS -: Peroxiredoxins are a family of thiol-dependent antioxidant proteins that catalyze the reduction of hydrogen peroxide, peroxynitrite and a wide range of organic hydroperoxides (ROOH) to the corresponding alcohol (ROH) and water. These proteins function as peroxidases only when coupled to a sulfhydryl-reducing system such as thioredoxin or glutathione. Catalytic activity is dependent on the oxidation of a conserved cysteine residue at the active site. (Adapted from FBrf0139661, PMID:12517450.)

Protein Function (UniProtKB)

(UniProt, Q9V3P0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Prx2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V3P0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Prx2-RA

FBtr0073764

NM_058162

1010

194

Prx2-RB

FBtr0073763

NM_167359

1014

194

Prx2-RC

FBtr0339699

NM_001298273

1051

194

Prx2-RD

FBtr0339700

NM_001298274

1080

194

Prx2-RE

FBtr0345161

NM_001298275

976

194

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Prx2-PA

FBpp0073595

21.7

194

5.34

FBpp0073594

21.7

194

5.34

FBpp0308756

21.7

194

5.34

FBpp0308757

21.7

194

5.34

FBpp0311371

21.7

194

5.34

Q9V3P0

NP_001285204

AHN59674

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

194 aa isoforms: Prx2-PA, Prx2-PB, Prx2-PC, Prx2-PD, Prx2-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer; disulfide-linked, upon oxidation (PubMed:33920774). 5 homodimers assemble to form a ring-like decamer (By similarity). Also exists as a monomer (PubMed:33920774).

(UniProt, Q9V3P0)

Post Translational Modification

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.

Conjugated to URM1, a ubiquitin-like protein.

(UniProt, Q9V3P0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prx2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.77

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 1 -- 5

organism | ubiquitous | uniform

Comment: maternally deposited

(Degennaro et al., 2011)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 6

primordial germ cell

(Degennaro et al., 2011)

embryonic stage 7 -- 8

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9

germline cell

(Degennaro et al., 2011)

embryonic stage 9 -- 10

germline cell

(Fisher et al., 2012)

embryonic stage 11 -- 12

germline cell

(Fisher et al., 2012)

lymph gland primordium

Comment: reported as lymph gland specific anlage

(Fisher et al., 2012)

procrystal cell

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic precursor of adult hindgut

(Fisher et al., 2012)

embryonic/larval crystal cell

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic hypopharynx

(Fisher et al., 2012)

embryonic/larval corpus allatum

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic optic lobe primordium

(Fisher et al., 2012)

embryonic stage 14

embryonic/larval crystal cell

(Degennaro et al., 2011)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | intense

(Radyuk et al., 2001)

third instar larval stage

organism

(Radyuk et al., 2001)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

organism

(Lee et al., 2009)

third instar larval stage

larval brain

(Lee et al., 2009)

imaginal disc

(Lee et al., 2009)

embryonic/larval Malpighian tubule

(Lee et al., 2009)

(Lee et al., 2009)

(Lee et al., 2009)

Additional Descriptive Data

Prx2 transcript is maternally deposited. Maternal transcript quickly degrades, except in germline cells, where it is protected from degradation through embryonic stage 9. At later embryonic stages, Prx2 transcript is expressed in crystal cells.

(Degennaro et al., 2011)

Prx2 transcripts are detected throughout deveopment by RT-PCR and are abundant in third instar larval brain, imaginal discs and Malpighian tubules.

(Lee et al., 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 5

somatic cell

• cytosol

(Degennaro et al., 2011)

primordial germ cell

• cytosol

(Degennaro et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Radyuk et al., 2001)

day 1 of adulthood | male -- day 2 of adulthood | male

adult thorax

• cytosol

(Radyuk et al., 2001)

Additional Descriptive Data

Prx2 is present throughout blastoderm embryos in both somatic cells and germline precursor cells.

(Degennaro et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(Radyuk et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Prx2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Prx2

View exonic expression by tissue for Dmel\Prx2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040309

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53578/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040309

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040309

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040309

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1633

Flygut - An atlas of the Drosophila adult midgut

FBgn0040309

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Prx2

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Prx2

Transgenic constructs containing regulatory region of Prx2

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(1)Exel6245

(Ryder, 2004.4.26)

Gene not disrupted in

Df(1)f08066-f07791

(Casad et al., 2011)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Other Phenotypes

Allele

abnormal cell adhesion

abnormal cell adhesion (with Prx2^f08066)

Prx2^KG05372

abnormal cell adhesion (with Prx2^KG05372)

Prx2^f08066

abnormal cell migration

abnormal cell migration (with Prx2^f08066)

Prx2^KG05372

abnormal cell migration (with Prx2^KG05372)

Prx2^f08066

abnormal chemosensory behavior, with Scer\GAL4^da.PU

Prx2^UAS.cHa

abnormal oxidative stress response

Prx2^KG05372

abnormal oxidative stress response | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

Prx2^UASp.cDa

abnormal starvation stress response

Prx2^KG05372

abnormal starvation stress response, with Scer\GAL4^NP5130

Prx2^UAS.cBa

long lived, with Scer\GAL4^NP5130

Prx2^UAS.cBa

short lived | conditional, with Scer\GAL4^da.G32

Prx2^UAS.cHa

Phenotype manifest in

Allele

germline cell | maternal effect

germline cell | maternal effect (with Prx2^f08066)

Prx2^KG05372

germline cell | maternal effect (with Prx2^KG05372)

Prx2^f08066

midgut | adult stage, with Scer\GAL4^NP5130

Prx2^RNAi.UAS.cUa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (6)

Hsap\PRDX2

12 of 14

Yes

Hsap\PRDX1

10 of 14

Yes

8 of 14

7 of 14

3 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Prdx2

12 of 14

Yes

Rnor\Prdx1

11 of 14

Yes

Rnor\Prdx1l1

9 of 14

Yes

Rnor\Prdx4

8 of 14

Rnor\Prdx3

7 of 14

Rnor\Prdx6

3 of 14

Mus musculus (laboratory mouse) (6)

Mmus\Prdx2

11 of 14

Yes

Mmus\Prdx1

9 of 14

Yes

8 of 14

7 of 14

3 of 14

3 of 14

Xenopus tropicalis (Western clawed frog) (7)

Xtro\prdx2

10 of 13

Yes

Xtro\prdx1

3 of 13

Yes

Xtro\prdx4

3 of 13

Xtro\limk2

2 of 13

Xtro\lemd1

1 of 13

Yes

Xtro\prdx3

1 of 13

Xtro\prdx6

1 of 13

Danio rerio (Zebrafish) (5)

Drer\prdx2

12 of 14

Yes

Drer\prdx1

11 of 14

Yes

Drer\prdx4

8 of 14

Drer\prdx3

7 of 14

Drer\prdx6

3 of 14

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\prdx-2

12 of 14

Yes

Cele\prdx-3

8 of 14

Cele\prdx-6

3 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\TPX2

12 of 12

Yes

6 of 12

6 of 12

2 of 12

2 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\2-Cys Prx B

12 of 13

Yes

Atha\AT3G11630

12 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\TSA1

13 of 13

Yes

Scer\TSA2

12 of 13

Yes

Scer\PRX1

3 of 13

Scer\DOT5

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\tpx1

12 of 12

Yes

Spom\but1

1 of 12

Escherichia coli (enterobacterium) (2)

Ecol\ahpC

10 of 11

Yes

Ecol\bcp

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Prx2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

11 of 13

11 of 13

10 of 13

4 of 13

4 of 13

4 of 13

4 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PRDX1; peroxiredoxin 1

model of methylmalonic aciduria and homocystinuria type cblC

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PRDX2; peroxiredoxin 2

12 of 14

600538

PRDX1; peroxiredoxin 1

10 of 14

176763

METHYLMALONIC ACIDURIA AND HOMOCYSTINURIA, cblC TYPE; MAHCC

methylmalonic aciduria and homocystinuria type cblC

PRDX4; peroxiredoxin 4

8 of 14

300927

PRDX3; peroxiredoxin 3

7 of 14

PEROXIREDOXIN 3; PRDX3

autosomal recessive spinocerebellar ataxia 32

PRDX6; peroxiredoxin 6

3 of 14

602316

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Prx2 - Mekk1

anti tag coimmunoprecipitation, disulfide bond, anti tag western blot

(Barata and Dick, 2020)

Prx2 - MtnA

pull down, anti tag western blot

(Tio et al., 2009)

Prx2 - MtnB

two hybrid, pull down, anti tag western blot

(Tio et al., 2009)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

gfzf

suppressible

Prx2

(Smith et al., 2019)

suppressible

(Nam et al., 2012)

suppressible

(Nam et al., 2012)

enhanceable

(Degennaro et al., 2011)

Urm1

suppressible

Prx2

(Khoshnood et al., 2016)

External Data

Subunit Structure (UniProtKB)

Homodimer; disulfide-linked, upon oxidation (PubMed:33920774). 5 homodimers assemble to form a ring-like decamer (By similarity). Also exists as a monomer (PubMed:33920774).

(UniProt, Q9V3P0 )

Linkouts

BioGRID - A database of protein and genetic interactions.

72802

DroID - A comprehensive database of gene and protein interactions.

FBgn0040309

MIST (genetic) - An integrated Molecular Interaction Database

53578

MIST (protein-protein) - An integrated Molecular Interaction Database

53578

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Peroxisome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-42

Cytogenetic map

11E11-11E11

Sequence location

X:13,329,824..13,331,994 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11E11-11E11

Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640^EP1626

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

11E-11E

(Santaren, 1999.7.8)

11E6-11E8

(Radyuk, 2000.11.16)

11E-11E

(determined by in situ hybridisation)

(Rodriguez et al., 2000)

Experimentally Determined Recombination Data

Location

1-42

(FlyBase, 2016)

1-42.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

14440

y¹ P{SUPor-P}Prx2^KG05372

32498

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00501}attP2

34971

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00935}attP2

38926

y¹ sc^* v¹ sev²¹; P{TRiP.GL01018}attP40

Kyoto

112979

w^* P{GawB}Prx2^NP2597 / FM7c

104253

y^* w^* P{GawB}Prx2^NP2597 / FM7c

113854

y^* w^* P{GawB}Prx2^NP6118 / FM7c

NIG-Fly

1633R-3

P{NIG.1633R}3

HMS00501

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00501}attP2

VDRC

v330046

P{VSH330046}attP40

v109514

P{KK110159}VIE-260B

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (136)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040309

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040309

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Degennaro et al., 2011, Radyuk et al., 2001)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : Stable S2 cell lines were created to over-express Prx3 or Prx2.
(Radyuk et al., 2003)

Other Comments

Jafrac1 regulates germ cell internalization during gastrulation.

(Degennaro et al., 2011)

Jafrac1 protects flies from oxidative stress.

(Degennaro et al., 2011)

Jafrac1 is not required for the formation, specification, motility, or survival of primordial germline cells.

(Degennaro et al., 2011)

Trx-2 not dhd is the substrate of Jafrac1.

(Bauer et al., 2002)

Relationship to Other Genes

Source for database merge of

Source for merge of: Jafrac1 CG1633

(Bayraktaroglu, 2000.8.7)

Additional comments

'CG6888' (Prx1) and 'CG1633' (Prx2) are paralogs and are homologous to both mammalian Prx1 and Prx2.

(McGinnis et al., 2021)

The CG6888 gene may have been derived from the Jafrac1 gene by retroposition.

(Dai et al., 2006)

Relationship between "Jafrac1" and "SSP6107" is unclear; they may represent alternative transcripts of the same gene, or two separate genes.

(Rodriguez et al., 2000)

Nomenclature History

Source for database identify of

Source for identity of: Jafrac1 Prx4783

(Orr, 2001.3.5)

Source for identity of: Prx2 Jafrac1

(McGinnis et al., 2021)

Nomenclature comments

Etymology

'CG1633' is a homolog of mammalian Prx1 or Prx2, but is more closely related to mammalian Prx2 based on the features of the amino acid sequence. Thus it is named as 'Prx2'.

(McGinnis et al., 2021)

The gene is named "Jafrac1" after the initials of people in the Santaren research group who first published on the gene.

(Santaren, 2001.10.24)

Synonyms and Secondary IDs (36)

Reported As

Symbol Synonym

1633

(Ravi et al., 2009)

DPx-4783

(Radyuk et al., 2003, Orr and Radyuk, 2002, Radyuk et al., 2001)

DPx4783

(Detienne et al., 2018, Heifetz and Rivlin, 2010, Orr et al., 2001)

DmTPx-1

(Bauer et al., 2002)

Dpx-4783

(Lee et al., 2009)

JAFRAC 1

(Abolaji et al., 2015)

Jafrac

(Saucedo et al., 2021, Pridie et al., 2020, Besson et al., 2015, Gao et al., 2014)

Jafrac1

(Tsuji et al., 2024, Vesala et al., 2024, Wang et al., 2024, Deng et al., 2023, Zhang et al., 2023, Fasae and Abolaji, 2022, Liu et al., 2022, Ogunsuyi et al., 2022, Ogunsuyi et al., 2022, Gan et al., 2021, Girard et al., 2021, Michki et al., 2021, Sureka and Mishra, 2021, Toh et al., 2021, Olufs et al., 2020, Rodriguez-Fernandez et al., 2020, Vásquez-Procopio et al., 2020, Bradshaw, 2019, Hall et al., 2019, Smith et al., 2019, Anderson-Baron and Simmonds, 2018, Jo and Imm, 2017, Khoshnood et al., 2017, McClelland et al., 2017, Miller et al., 2017, Wang et al., 2017, Du et al., 2016, Khoshnood et al., 2016, Besson et al., 2015, Dent et al., 2015, Izutsu et al., 2015, Kavi et al., 2015, Omelyanchuk et al., 2015, Ugrankar et al., 2015, Ashwal-Fluss et al., 2014, Huang et al., 2014, Luck et al., 2014, Mukherjee et al., 2014, Wang et al., 2014, Colinet et al., 2013, Kockmann et al., 2013, Kwon et al., 2013, Mishra et al., 2013, Sen et al., 2013, Edgar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Albrecht et al., 2011, Degennaro et al., 2011, Hochmuth et al., 2011, Wangler et al., 2011, Biteau et al., 2010, Gallach et al., 2010, Monzo et al., 2010, Müller et al., 2010, Lee et al., 2009, Li et al., 2009, Tio et al., 2009, Curtis et al., 2007, Lee et al., 2007, Stuart et al., 2007, Radyuk et al., 2001)

PRDX1

(Anderson-Baron and Simmonds, 2018)

PRDX1/2/4

(Baron et al., 2016)

PRDX1_DROME

(Fukuyama et al., 2012)

Prx

(Dolezal, 2023, Tio et al., 2009)

Prx2

(Rabah et al., 2025, Salminen et al., 2024, Scharenbrock et al., 2024, Nozawa et al., 2023)

Prx4783

SSP5111

(Rodriguez et al., 2000)

T20

(Vierstraete et al., 2004)

TPX-1

(Lotz et al., 2014)

cTPx

(Radyuk et al., 2003)

dPRDX2

(Murari et al., 2022)

dPrx I

(Chen et al., 2005)

dPrx1

(Bradshaw, 2019, Detienne et al., 2018)

dPrx2

(McGinnis et al., 2021)

jafrac1

(Nam et al., 2012, Degennaro et al., 2011, Biteau et al., 2010, Lee et al., 2007, Ha et al., 2005, Ha et al., 2005)

jf1

(Degennaro et al., 2011)

Name Synonyms

Jafrac-1

(Kuranaga, 2011)

Jafrac1

(Detienne et al., 2018, Heifetz and Rivlin, 2010)

Peroxiredoxin

(Castillo et al., 2015)

Peroxiredoxin 1

(Anholt and Williams, 2010, Pflieger et al., 2008)

Peroxiredoxin 2

(Farodoye et al., 2024)

Peroxiredoxin 4783

(Orr et al., 2001)

Peroxiredoxin I

(Chen et al., 2005)

Thioredoxin peroxidase 1

(Anderson-Baron and Simmonds, 2018, Heifetz and Rivlin, 2010, de Morais Guedes et al., 2003)

jafrac1

(Biteau et al., 2011)

peroxidase

(Curtis et al., 2007)

peroxiredoxin

(Tio et al., 2009)

thioredoxin peroxidase

(Zhang et al., 2004)

thioredoxin peroxidase 1

(Ramond et al., 2020, Detienne et al., 2018, Jaspers et al., 2012, Cammarato et al., 2011, Santaren, 1999.7.8)

Secondary FlyBase IDs

FBgn0030472

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 66 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53578

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V3P0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V3P0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2JF59

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V3P0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040309

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53578/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040309

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040309

FlyMine - An integrated database for Drosophila genomics

FBgn0040309

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1633

MARRVEL_MODEL - MARRVEL (model organism gene)

53578

Linkouts

BioGRID - A database of protein and genetic interactions.

72802

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040309

DroID - A comprehensive database of gene and protein interactions.

FBgn0040309

DRSC - Results frm RNAi screens

FBgn0040309

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040309

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040309

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1633

Flygut - An atlas of the Drosophila adult midgut

FBgn0040309

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0040309

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Peroxisome

MIST (genetic) - An integrated Molecular Interaction Database

53578

MIST (protein-protein) - An integrated Molecular Interaction Database

53578

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (160)

The following external sites may use different assemblies or annotations than FlyBase.

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