Jafrac1
SBP tag
Mekk1
Myc tag
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Under non-reducing conditions, multiple Mekk1-containing species co-precipitate with Jafrac1 while under reducing conditions only monomeric Mekk1 co-precipitates suggesting both covalent and non-covalent interactions.
Under non-reducing conditions, multiple Jafrac1-containing species co-precipitate with Mekk1.