FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rho1, RhoGEF2
FlyBase ID
FBig0000115949
Interaction Network
Interactions Browser links
Rho1 network
RhoGEF2 network
Reported Interactions
FBrf0237354-1.PD.A
Reference
Nakamura et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait

GST tag

RhoGEF2 

RhoGEF2

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Nakamura et al., 2017)
FBrf0254509-1.PD.WB
Reference
Lin et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait

GST tag

RhoGEF2 

RhoGEF2

prey

biotin label

RhoGEF2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RhoGEF2 
PH domain residue 1842
mutation decreasing interaction
aa 1842

I1842E; within the context of aa 1770-1878.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lin et al., 2022)

Interaction between Rho1 and RhoGEF2 equivalent with either Rho1 bound to GDP or GFP.

(Lin et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)