FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000115973
Interaction Network
Interactions Browser links
His3 network
E(bx) network
Reported Interactions
FBrf0195919-1.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

NURF301

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide source unspecified; prey derived from Nurf complex reconstituted from proteins expressed in a baculovirus and Sf9 cell system.

FBrf0195919-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

NURF301

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide source unspecified; prey derived from Nurf complex reconstituted from proteins expressed in a baculovirus and Sf9 cell system.

E(bx) preferentially binds K4me3 modified version of His3.

FBrf0195919-6.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

NURF301

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PHD2 domain
sufficient binding region
aa 2490-2553

coordinates relative to E(bx)-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

E(bx) preferentially binds K4me3 modified version of His3.

Interaction in vitro; bait peptide source unspecified; prey produced as a recombinant fusion protein in bacterial system.

FBrf0208433-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

biotin tag

His3 

Nurf301

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His3 
N-terminal domain
sufficient binding region
aa 1-21
PHD domain
sufficient binding region
aa 2490-2553
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide produced commercially; prey produced as a recombinant fusion protein in bacterial system.

FBrf0208433-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Nurf301

bait

GST tag

His3 

H3

prey

biotin tag

His3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His3 
N-terminal domain
sufficient binding region
aa 1-21
PHD domain
sufficient binding region
aa 2490-2553
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide produced commercially.

FBrf0208546-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

biotin tag

His3 

dNURF301

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His3 
N-terminal domain
sufficient binding region
aa 1-21
PHD domain
sufficient binding region
aa 2488-2567

coordinates relative to E(bx)-PA

PHD domain residue 2519
mutation disrupting interaction
aa 2519

W2519A or W2519F, coordinates relative to E(bx)-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptides produced commercially; prey produced as a recombinant fusion protein in bacterial system.

The His3-E(bx) interaction is stronger with H3K4me3 than H3K4me2.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)