Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2R+ cells.

Treatment of cells with spi increases the Graf-Egfr interaction. Knockdown of Cbl via dsRNA or the Y1271F Egfr mutation decreases the spi induced Graf-Egfr interaction but has no effect on the basal Graf-Egfr interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Treatment of cells with spi increases the Graf-Egfr interaction. Knockdown of Cbl via dsRNA or the Y1271F Egfr mutation decreases the spi induced Graf-Egfr interaction but has no effect on the basal Graf-Egfr interaction.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The addition of a ubiquitin tag to Egfr increases the Graf-Egfr interaction.