FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116050
Interaction Network
Interactions Browser links
Atu network
Myc network
Reported Interactions
FBrf0237119-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atu 

Leo1

bait

HA tag

Myc 

Myc

prey

HA tag

Myc 
Myc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Myc 
interaction domain
sufficient binding region
aa 1-525aa 294-717
Myc 
interaction domain
necessary binding region
aa 404-525
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0237119-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atu 

Leo1

bait

AU1 tag

Atu 
Atu 
Myc 

Myc

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atu 
interaction domain
sufficient binding region
aa 1-471aa 371-725
Atu 
interaction domain
necessary binding region
aa 442-474
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0237119-3.PLA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atu 

Leo1

neutral component

HA tag

Atu 

Leo1

neutral component

HA tag

Atu 

Leo1

neutral component

HA tag

Myc 

Myc

neutral component
Myc 

Myc

neutral component
Myc 

Myc

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; proteins produced from endogenous gene or from transfected construct.

The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).

FBrf0237119-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atu 

Leo1

bait

HA tag

Atu 
Atu 
Myc 

Myc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atu 
interaction domain
sufficient binding region
aa 1-471aa 371-725
Atu 
interaction domain
necessary binding region
aa 442-474
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0237119-5.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Myc 

Myc

bait

GST tag

Atu 

Leo1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)