FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
key, Atg8a
FlyBase ID
FBig0000116104
Interaction Network
Interactions Browser links
key network
Atg8a network
Reported Interactions
FBrf0237111-1.PD.A
Reference
Tusco et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg8a 

Atg8a

bait

GST tag

Atg8a 
key 

Kenny

prey

[35]S label, Myc tag

key 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
key 
LIR domain
mutation disrupting interaction
aa 7,10

F7A,L10A

Atg8a 
LDS domain
mutation disrupting interaction
aa 48,49

K48A,Y49A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Tusco et al., 2017)
FBrf0237111-2.coIP.WB
Reference
Tusco et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
key 

Kenny

bait

FLAG tag

key 
Atg8a 

Atg8a

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
key 
LIR domain
mutation decreasing interaction
aa 7,10

F7A,L10A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tusco et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)