Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult head cDNA expression library).
Two-hybrid system: yeast LexA-BD/B42-AD
Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.