FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ssx, msl-2
FlyBase ID
FBig0000116202
Interaction Network
Interactions Browser links
ssx network
msl-2 network
Reported Interactions
FBrf0237805-1.coIP.PCR
Reference
Moschall et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ssx 

Ssx

bait

FLAG tag

msl-2 

msl-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Moschall et al., 2018)
FBrf0237805-2.EMSA.A
Reference
Moschall et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ssx 

Ssx

bait

GST tag

ssx 
msl-2 

msl-2

prey

[32]P label

msl-2 
msl-2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
msl-2 
5-prime UTR
sufficient binding region
msl-2 
5-prime UTR
mutation disrupting interaction
UUUUUUUUUUUUUUUU

mutated to CUCUCUCUCUCUCUCU

ssx 
RBD4 domain
sufficient binding region
aa 93-269
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Moschall et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)