FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
x16, Doa
FlyBase ID
FBig0000116209
Interaction Network
Interactions Browser links
x16 network
Doa network
Reported Interactions
FBrf0202595-3.Y2H
Reference
Wan et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
x16 

dx16

bait

fused to GAL4 DNA-binding domain

Doa 

doa

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Wan et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Wan et al., 2008)
FBrf0202595-6.PD.WB
Reference
Wan et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Doa 

doa

bait

GST tag

Doa 
Doa 
Doa 
x16 

dx16

prey

His tag

x16 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
x16 
RS domain
sufficient binding region
unspecified
Doa 
interaction domain 1
sufficient binding region
aa 28-99
Doa 
interaction domain 2
sufficient binding region
aa 94-169
Doa 
interaction domain 3
sufficient binding region
aa 480-517
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Wan et al., 2008)
FBrf0202595-7.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Doa 

doa

enzyme
Doa 

doa

enzyme
x16 

dx16

enzyme target

P[32] label

x16 

dx16

enzyme target

P[32] label

x16 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)