FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\x16
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General Information
Symbol
Dmel\x16
Species
D. melanogaster
Name
x16 splicing factor
Annotation Symbol
CG10203
Feature Type
protein_coding_gene
FlyBase ID
FBgn0028554
Gene Model Status
Current
Stock Availability
20 publicly available
Gene Summary
x16 splicing factor (x16) encodes a protein involved in RNA splicing and RNA metabolism regulation. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)
All Summaries
Also Known As

9G8, xl6, Dxl6, l(2)k00230, d9G8

Key Links
Genomic Location
Cytogenetic map
27C4-27C4
Sequence location
Recombination map
2-23
RefSeq locus
NT_033779 REGION:6914301..6920210
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables mRNA binding
inferred from direct assay
(Bradley et al., 2015)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables mRNA binding
inferred from biological aspect of ancestor with PANTHER:PTN009063129
(GO Reference Genome Project, 2011-)
enables nucleic acid binding
inferred from electronic annotation with InterPro:IPR001878, InterPro:IPR035979, InterPro:IPR036875
(InterPro Project Members, 2004-)
enables RNA binding
inferred from sequence model
(Vorbruggen et al., 2000)
inferred from electronic annotation with InterPro:IPR000504
(InterPro Project Members, 2004-)
enables zinc ion binding
inferred from electronic annotation with InterPro:IPR001878, InterPro:IPR036875
(InterPro Project Members, 2004-)
Biological Process (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in regulation of alternative mRNA splicing, via spliceosome
inferred from mutant phenotype
(Bradley et al., 2015)
involved_in regulation of gene expression
inferred from mutant phenotype
(Bradley et al., 2015)
involved_in regulation of mRNA 3'-end processing
inferred from mutant phenotype
(Bradley et al., 2015)
involved_in regulation of transcriptional start site selection at RNA polymerase II promoter
inferred from mutant phenotype
(Bradley et al., 2015)
involved_in RNA splicing
(Wan et al., 2008)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in mRNA splicing, via spliceosome
inferred from sequence or structural similarity with UniProtKB:P84103
(Vorbruggen et al., 2000)
inferred by curator from GO:0071011,GO:0071013
(Herold et al., 2009)
involved_in regulation of alternative mRNA splicing, via spliceosome
inferred from biological aspect of ancestor with PANTHER:PTN009063129
(GO Reference Genome Project, 2011-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
part_of catalytic step 2 spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
located_in nucleus
inferred from direct assay
(Wan et al., 2008, Allemand et al., 2002)
part_of precatalytic spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nuclear speck
inferred from biological aspect of ancestor with PANTHER:PTN000567445
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
x16 splicing factor (x16) encodes a protein involved in RNA splicing and RNA metabolism regulation. [Date last reviewed: 2019-09-19]
Gene Group (FlyBase)
CANONICAL SERINE AND ARGININE RICH SPLICING FACTORS -
Canonical serine and arginine rich splicing factors contain 1-2 RNA-binding motifs and an RS domain. They are involved in the recruitment of spliceosomal components, interacting with pre-mRNA exonic sequences to regulate splice site selection. (Adapted from FBrf0243536.)
Protein Function (UniProtKB)
Serine/arginine-rich splicing factor (SR protein) involved in differential exon usage during RNA transcript processing, probably by binding exonic splicing enhancer elements and recruiting components of the splicing machinery (PubMed:17345156, PubMed:35780586). Binds RNA stem-loop structures with consensus sequence 5'-CCGUNUNKNW-3' (PubMed:17345156). Regulator of genes involved in lipid and carbohydrate metabolism, the immune response and the response to xenobiotics (PubMed:35780586).
(UniProt, Q9V3V0)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\x16 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9V3V0)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.55

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
x16-RA
FBtr0079368
NM_164718
1707
258
x16-RB
FBtr0332328
NM_001273239
1784
257
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
x16-PA
FBpp0078996
27.9
258
12.06
Q9V3V0
NP_723226
AAF52454
x16-PB
FBpp0304606
27.7
257
12.03
M9PC85
NP_001260168
AGB92704
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

258 (aa)

(Vorbruggen et al., 2000)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts (via Arg/Ser-rich region) with Alsin2/CG7564, Rbp1 and Doa (via N-terminus).

(UniProt, Q9V3V0)
Post Translational Modification

Highly phosphorylated (PubMed:17828581). May be phosphorylated by the serine/threonine-protein kinase Doa (PubMed:17828581).

(UniProt, Q9V3V0)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\x16 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.34

Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 5 -- 11
organism | ubiquitous
(Vorbruggen et al., 2000)
embryonic stage 14 -- 16
presumptive embryonic/larval central nervous system
(Vorbruggen et al., 2000)
third instar larval stage
leg disc | restricted
(Vorbruggen et al., 2000)
eye-antennal disc | restricted
(Vorbruggen et al., 2000)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11
ventral neurectoderm
(Vorbruggen et al., 2000)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
larval lateral VNC glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
embryonic stage 14 -- 16
embryonic brain
(Vorbruggen et al., 2000)
third instar larval stage
wing disc | restricted
(Vorbruggen et al., 2000)
adult stage & oogenesis
nurse cell
(Vorbruggen et al., 2000)
Additional Descriptive Data

x16 transcripts are first detected during oogenesis, primarily in the nurse cells. As oogenesis proceeds, transcripts are detected in the oocyte, indicating that they are transported from the nurse cells to the oocyte. x16 transcripts are detected ubiquitously in early embryos, but localized expression is detected following the onset of zygotic expression. By embryonic stage 11 transcripts are detected in the neurectoderm and become increasingly localized to the central nervous system until the completion of embryogenesis. During larval stages, transcripts are expressed at low levels in a ubiquitous pattern, but in the imaginal discs, localized expression is noted.

(Vorbruggen et al., 2000)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 5
organism | ubiquitous
(Wan et al., 2008)
embryonic stage 14 -- 16
larval central nervous system
(Wan et al., 2008)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

x16 protein is ubiquitously expressed at embryonic stage 5. At later embryonic stages, it is highly expressed in the CNS.

(Wan et al., 2008)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of catalytic step 2 spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
located_in nucleus
inferred from direct assay
(Wan et al., 2008, Allemand et al., 2002)
part_of precatalytic spliceosome
inferred from high throughput direct assay
(Herold et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\x16 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 12 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of x16
Transgenic constructs containing regulatory region of x16
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (48)
Hsap\SRSF7
12 of 14
Yes
Yes
1  
Hsap\SRSF3
9 of 14
No
Yes
2  
Hsap\RBMY1A1
2 of 14
No
Yes
Hsap\RBMY1D
2 of 14
No
Yes
Hsap\RBMY1J
2 of 14
No
Yes
Hsap\SRSF10
2 of 14
No
Yes
Hsap\SRSF12
2 of 14
No
Yes
Hsap\A1CF
1 of 14
No
No
Hsap\BOLL
1 of 14
No
No
2  
Hsap\CACNA1E
1 of 14
No
No
Hsap\CIRBP
1 of 14
No
No
Hsap\CSTF2
1 of 14
No
No
Hsap\CSTF2T
1 of 14
No
No
Hsap\ELAVL4
1 of 14
No
No
2  
Hsap\G3BP1
1 of 14
No
No
1  
Hsap\NCBP2
1 of 14
No
No
Hsap\NCBP2L
1 of 14
No
No
Hsap\NONO
1 of 14
No
No
4  
Hsap\PABPC1
1 of 14
No
No
2  
Hsap\PABPC1L
1 of 14
No
No
Hsap\PABPN1
1 of 14
No
No
8  
Hsap\PPIE
1 of 14
No
No
Hsap\PSPC1
1 of 14
No
No
1  
Hsap\RBM3
1 of 14
No
No
Hsap\RBM4B
1 of 14
No
No
Hsap\RBM4
1 of 14
No
No
1  
Hsap\RBM14
1 of 14
No
No
Hsap\RBM14-RBM4
1 of 14
No
No
Hsap\RBM19
1 of 14
No
No
1  
Hsap\RBMX
1 of 14
No
No
Hsap\RBMXL1
1 of 14
No
No
Hsap\RBMY1B
1 of 14
No
No
Hsap\RBMY1C
1 of 14
No
Yes
Hsap\RBMY1E
1 of 14
No
Yes
Hsap\RBMY1F
1 of 14
No
Yes
Hsap\SF3B6
1 of 14
No
No
Hsap\SFPQ
1 of 14
No
No
3  
Hsap\SRSF1
1 of 14
No
No
20  
Hsap\SRSF2
1 of 14
No
No
0  
Hsap\SRSF4
1 of 14
No
No
1  
Hsap\SRSF5
1 of 14
No
No
1  
Hsap\SRSF6
1 of 14
No
No
1  
Hsap\SRSF8
1 of 14
No
No
Hsap\SRSF9
1 of 14
No
No
1  
Hsap\TIAL1
1 of 14
No
No
1  
Hsap\TRA2A
1 of 14
No
No
2  
Hsap\TRA2B
1 of 14
No
No
1  
Hsap\ZCRB1
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (28)
Rnor\Srsf7
12 of 14
Yes
Yes
Rnor\Srsf3
9 of 14
No
Yes
Rnor\Srsf10
2 of 14
No
Yes
Rnor\Srsf12
2 of 14
No
Yes
Rnor\Cirbp
1 of 14
No
No
Rnor\Cstf2
1 of 14
No
No
Rnor\Elavl4
1 of 14
No
No
Rnor\Ncbp2
1 of 14
No
No
Rnor\Pabpc1
1 of 14
No
No
Rnor\Pabpc4l
1 of 14
No
No
Rnor\Pabpc6
1 of 14
No
No
Rnor\Ppie
1 of 14
No
No
Rnor\Rbm3
1 of 14
No
No
Rnor\Rbm4b
1 of 14
No
No
Rnor\Rbmxl1b
1 of 14
No
No
Rnor\Rbmx
1 of 14
No
No
Rnor\Rbmy1j
1 of 14
No
No
Rnor\Srsf1
1 of 14
No
No
Rnor\Srsf2
1 of 14
No
No
Rnor\Srsf4
1 of 14
No
No
Rnor\Srsf5
1 of 14
No
No
Rnor\Srsf6
1 of 14
No
No
Rnor\Srsf8
1 of 14
No
No
Rnor\Srsf9
1 of 14
No
No
Rnor\Tial1
1 of 14
No
No
Rnor\Tra2a
1 of 14
No
No
Rnor\Tra2b
1 of 14
No
No
Rnor\Zcrb1
1 of 14
No
Yes
Mus musculus (laboratory mouse) (36)
Mmus\Srsf7
12 of 14
Yes
Yes
Mmus\Srsf3
9 of 14
No
Yes
Mmus\4930595M18Rik
1 of 14
No
No
Mmus\Cirbp
1 of 14
No
No
Mmus\Cstf2
1 of 14
No
No
Mmus\Elavl4
1 of 14
No
No
Mmus\Gm3376
1 of 14
No
No
Mmus\Gm4064
1 of 14
No
No
Mmus\Gm10256
1 of 14
No
Yes
Mmus\Gm10352
1 of 14
No
No
Mmus\Gm21677
1 of 14
No
No
Mmus\Gm21693
1 of 14
No
No
Mmus\Gm21704
1 of 14
No
No
Mmus\Gm21708
1 of 14
No
No
Mmus\Ncbp2
1 of 14
No
No
Mmus\Pabpc1
1 of 14
No
No
Mmus\Pabpc4l
1 of 14
No
No
Mmus\Pabpc6
1 of 14
No
No
Mmus\Ppie
1 of 14
No
No
Mmus\Rbm3
1 of 14
No
No
Mmus\Rbm4b
1 of 14
No
No
Mmus\Rbm4
1 of 14
No
No
Mmus\Rbm28
1 of 14
No
No
Mmus\Rbmy
1 of 14
No
No
Mmus\Srsf1
1 of 14
No
No
Mmus\Srsf2
1 of 14
No
No
Mmus\Srsf4
1 of 14
No
No
Mmus\Srsf5
1 of 14
No
No
Mmus\Srsf6
1 of 14
No
No
Mmus\Srsf9
1 of 14
No
No
Mmus\Srsf10
1 of 14
No
Yes
Mmus\Srsf12
1 of 14
No
Yes
Mmus\Tial1
1 of 14
No
No
Mmus\Tra2a
1 of 14
No
No
Mmus\Tra2b
1 of 14
No
No
Mmus\Zcrb1
1 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (28)
Xtro\srsf7
9 of 13
Yes
Yes
Xtro\srsf3
7 of 13
No
Yes
Xtro\srsf10
2 of 13
No
Yes
Xtro\a1cf
1 of 13
No
No
Xtro\cirbp
1 of 13
No
No
Xtro\dazap1
1 of 13
No
No
Xtro\g3bp1
1 of 13
No
No
Xtro\hnrnpa3
1 of 13
No
No
Xtro\mrps9
1 of 13
No
No
Xtro\mthfsd
1 of 13
No
No
Xtro\ncbp2
1 of 13
No
No
Xtro\ndufs5
1 of 13
No
No
Xtro\pabpc1l
1 of 13
No
No
Xtro\raly
1 of 13
No
No
Xtro\rbm4b
1 of 13
No
No
Xtro\rbm19
1 of 13
No
No
Xtro\sf3b6
1 of 13
No
No
Xtro\snrnp70
1 of 13
No
No
Xtro\snx15
1 of 13
No
No
Xtro\srsf1
1 of 13
No
No
Xtro\srsf4
1 of 13
No
No
Xtro\srsf9
1 of 13
No
No
Xtro\srsf12
1 of 13
No
Yes
Xtro\tia1
1 of 13
No
No
Xtro\tra2a
1 of 13
No
No
Xtro\tra2b
1 of 13
No
No
Xtro\zcchc9
1 of 13
No
No
Xtro\zcrb1
1 of 13
No
Yes
Danio rerio (Zebrafish) (35)
Drer\srsf7a
12 of 14
Yes
Yes
Drer\srsf7b
9 of 14
No
Yes
Drer\srsf3b
8 of 14
No
Yes
Drer\srsf3a
2 of 14
No
No
Drer\cirbpa
1 of 14
No
No
Drer\cirbpb
1 of 14
No
No
Drer\ncbp2
1 of 14
No
No
Drer\nipblb
1 of 14
No
No
Drer\pabpc1a
1 of 14
No
No
Drer\pabpc1b
1 of 14
No
No
Drer\ppie
1 of 14
No
No
Drer\rbm4.1
1 of 14
No
No
Drer\rbm4.2
1 of 14
No
No
Drer\rbm4.3
1 of 14
No
No
Drer\rbm14a
1 of 14
No
No
Drer\rbm14b
1 of 14
No
No
Drer\rbm19
1 of 14
No
No
Drer\rbmx
1 of 14
No
Yes
Drer\srsf1a
1 of 14
No
No
Drer\srsf1b
1 of 14
No
No
Drer\srsf2a
1 of 14
No
No
Drer\srsf2b
1 of 14
No
No
Drer\srsf4
1 of 14
No
No
Drer\srsf5a
1 of 14
No
No
Drer\srsf5b
1 of 14
No
No
Drer\srsf6a
1 of 14
No
No
Drer\srsf6b
1 of 14
No
No
Drer\srsf9
1 of 14
No
No
Drer\srsf10b
1 of 14
No
Yes
Drer\tia1l
1 of 14
No
No
Drer\tra2a
1 of 14
No
No
Drer\tra2b
1 of 14
No
No
Drer\zcrb1
1 of 14
No
Yes
Drer\zgc:55733
1 of 14
No
No
Drer\zgc:77262
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (22)
Cele\rsp-6
10 of 14
Yes
Yes
Cele\Y57G11A.5
6 of 14
No
No
Cele\asd-1
1 of 14
No
No
Cele\cpf-2
1 of 14
No
No
Cele\grld-1
1 of 14
No
No
Cele\ncbp-2
1 of 14
No
No
Cele\pab-1
1 of 14
No
No
Cele\R06C1.4
1 of 14
No
No
Cele\rbm-3.1
1 of 14
No
No
Cele\rbm-3.2
1 of 14
No
No
Cele\rbm-42
1 of 14
No
No
Cele\rnp-1
1 of 14
No
No
Cele\rnp-4
1 of 14
No
No
Cele\rnp-5
1 of 14
No
No
Cele\rnp-7
1 of 14
No
No
Cele\rnp-9
1 of 14
No
No
Cele\rsp-1
1 of 14
No
No
Cele\rsp-2
1 of 14
No
No
Cele\rsp-3
1 of 14
No
No
Cele\rsp-4
1 of 14
No
No
Cele\rsp-5
1 of 14
No
No
Cele\tiar-1
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (28)
Agam\AgaP_AGAP008303
11 of 12
Yes
Yes
Agam\AgaP_AGAP007325
4 of 12
No
No
Agam\AgaP_AGAP009142
4 of 12
No
No
Agam\AgaP_AGAP000977
1 of 12
No
No
Agam\AgaP_AGAP001419
1 of 12
No
No
Agam\AgaP_AGAP001460
1 of 12
No
No
Agam\AgaP_AGAP001643
1 of 12
No
No
Agam\AgaP_AGAP002335
1 of 12
No
No
Agam\AgaP_AGAP002780
1 of 12
No
No
Agam\AgaP_AGAP003497
1 of 12
No
No
Agam\AgaP_AGAP004414
1 of 12
No
No
Agam\AgaP_AGAP004592
1 of 12
No
No
Agam\AgaP_AGAP005127
1 of 12
No
No
Agam\AgaP_AGAP006798
1 of 12
No
No
Agam\AgaP_AGAP007689
1 of 12
No
No
Agam\AgaP_AGAP008433
1 of 12
No
No
Agam\AgaP_AGAP009570
1 of 12
No
No
Agam\AgaP_AGAP009742
1 of 12
No
No
Agam\AgaP_AGAP009810
1 of 12
No
No
Agam\AgaP_AGAP009952
1 of 12
No
No
Agam\AgaP_AGAP010194
1 of 12
No
No
Agam\AgaP_AGAP010495
1 of 12
No
No
Agam\AgaP_AGAP010496
1 of 12
No
No
Agam\AgaP_AGAP010553
1 of 12
No
No
Agam\AgaP_AGAP010918
1 of 12
No
No
Agam\AgaP_AGAP011092
1 of 12
No
No
Agam\AgaP_AGAP013420
1 of 12
No
Yes
Agam\NCBP2_ANOGA
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (64)
Atha\RSZ21
11 of 13
Yes
Yes
Atha\RSZ22a
11 of 13
Yes
Yes
Atha\RSZ22
11 of 13
Yes
Yes
Atha\RS2Z32
3 of 13
No
Yes
Atha\RS2Z33
3 of 13
No
Yes
Atha\RS31a
2 of 13
No
No
Atha\RS31
2 of 13
No
No
Atha\RS40
2 of 13
No
No
Atha\RS41
2 of 13
No
No
Atha\AT1G60000
1 of 13
No
No
Atha\AT1G73530
1 of 13
No
Yes
Atha\AT2G21440
1 of 13
No
No
Atha\AT2G27330
1 of 13
No
Yes
Atha\AT2G43370
1 of 13
No
No
Atha\AT2G47310
1 of 13
No
No
Atha\AT3G07810
1 of 13
No
No
Atha\AT3G15010
1 of 13
No
No
Atha\AT3G46020
1 of 13
No
Yes
Atha\AT3G47120
1 of 13
No
No
Atha\AT4G12640
1 of 13
No
No
Atha\AT4G13860
1 of 13
No
Yes
Atha\AT4G26650
1 of 13
No
No
Atha\AT4G35785
1 of 13
No
No
Atha\AT5G04600
1 of 13
No
No
Atha\AT5G06210
1 of 13
No
Yes
Atha\AT5G19960
1 of 13
No
Yes
Atha\AT5G43960
1 of 13
No
No
Atha\AT5G47620
1 of 13
No
No
Atha\AT5G52545
1 of 13
No
No
Atha\AT5G55550
1 of 13
No
No
Atha\ATRZ-1A
1 of 13
No
No
Atha\AtRZ-1b
1 of 13
No
No
Atha\AtRZ-1c
1 of 13
No
Yes
Atha\CBP20
1 of 13
No
No
Atha\CP31B
1 of 13
No
No
Atha\CP33
1 of 13
No
No
Atha\CSDP1
1 of 13
No
No
Atha\CSTF64
1 of 13
No
No
Atha\CYP59
1 of 13
No
No
Atha\FCA
1 of 13
No
No
Atha\FPA
1 of 13
No
No
Atha\ML1
1 of 13
No
Yes
Atha\ML2
1 of 13
No
No
Atha\ML3
1 of 13
No
Yes
Atha\ML4
1 of 13
No
Yes
Atha\ML5
1 of 13
No
Yes
Atha\PAB2
1 of 13
No
No
Atha\PAB3
1 of 13
No
No
Atha\PAB4
1 of 13
No
No
Atha\PAB5
1 of 13
No
No
Atha\PHIP1
1 of 13
No
No
Atha\PSRP2
1 of 13
No
No
Atha\RBP45A
1 of 13
No
No
Atha\RBP45B
1 of 13
No
No
Atha\RBP47B
1 of 13
No
No
Atha\SC35
1 of 13
No
No
Atha\SCL30A
1 of 13
No
No
Atha\SR30
1 of 13
No
No
Atha\SR34a
1 of 13
No
No
Atha\SR34
1 of 13
No
No
Atha\SR34b
1 of 13
No
No
Atha\U1-70K
1 of 13
No
No
Atha\U11/U12-31K
1 of 13
No
Yes
Atha\UBP1A
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (11)
Scer\CBC2
1 of 13
Yes
No
Scer\HRB1
1 of 13
Yes
No
Scer\HRP1
1 of 13
Yes
No
Scer\IST3
1 of 13
Yes
No
Scer\MRD1
1 of 13
Yes
No
Scer\NGR1
1 of 13
Yes
No
Scer\NPL3
1 of 13
Yes
No
Scer\PUB1
1 of 13
Yes
No
Scer\RNA15
1 of 13
Yes
No
Scer\RNP1
1 of 13
Yes
Yes
Scer\TIF35
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (7)
Spom\SPBC428.12c
2 of 12
Yes
No
Spom\prp2
1 of 12
No
No
Spom\SPAC25G10.01
1 of 12
No
No
Spom\SPAC328.05
1 of 12
No
No
Spom\SPBC13A2.01c
1 of 12
No
No
Spom\SPBC1861.04c
1 of 12
No
No
Spom\srp2
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:x16. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (25)
Rbp1-like
10 of 13
Rbp1
10 of 13
Rsf1
7 of 13
SC35
3 of 13
SF2
3 of 13
B52
2 of 13
lark
2 of 13
maca
2 of 13
Cbp20
1 of 13
CG3335
1 of 13
CG4612
1 of 13
CstF64
1 of 13
cyp33
1 of 13
hfp
1 of 13
Hrb27C
1 of 13
mod
1 of 13
msi
1 of 13
nito
1 of 13
Pabp2
1 of 13
Ppil4
1 of 13
Rbp9
1 of 13
Rnp4F
1 of 13
rump
1 of 13
Sf3b6
1 of 13
tra2
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SRSF7; serine and arginine rich splicing factor 7
    12 of 14
    600572
         
        SRSF3; serine and arginine rich splicing factor 3
        9 of 14
        603364
             
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            TER94
            suppressible
            x16
            (Ritson et al., 2010)
            External Data
            Subunit Structure (UniProtKB)
            Interacts (via Arg/Ser-rich region) with Alsin2/CG7564, Rbp1 and Doa (via N-terminus).
            (UniProt, Q9V3V0 )
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2L
            Recombination map
            2-23
            Cytogenetic map
            27C4-27C4
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            27C4-27C4
            Limits computationally determined from genome sequence between P{PZ}Rca103300&P{lacW}l(2)k09022k09022 and P{lacW}xl6k00230&P{EP}Hrb27CEP748
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            27C4-27C5
            (Bourbon, 2000.2.9, Vorbrüggen, 1999.9.14)
            27C4-27C5
            (determined by in situ hybridisation)
            (Spradling et al., 1999, BDGP Project Members, 1994-1999)
            Experimentally Determined Recombination Data
            Location

            2-23

            (FlyBase, 2016)

            2-22.2

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (20)
            Genomic Clones (20)
            cDNA Clones (113)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              Antibody Information
              Laboratory Generated Antibodies
               
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                  Other Comments

                  Forced xl6 expression in the larval brain with a strong Scer\GAL4 driver caused remarkable increase of the number of neuroblasts in this region.

                  (Hirota et al., 2003)

                  Area matching Drosophila EST AA202757 (inverted).

                  (Davies, 2001.3.15)
                  Relationship to Other Genes
                  Source for database merge of

                  Source for merge of: xl6 9G8

                  (FlyBase, 1996-)

                  Source for merge of: xl6 anon-WO0118547.78

                  (FlyBase, 1996-)
                  Additional comments

                  snoRNA:nop5-x16-a is encoded in an intron of nop5 or x16.

                  (Graveley et al., 2011)

                  Source for merge of xl6 9G8 was sequence comparison ( date:000411 ).

                  (FlyBase, 1996-)

                  Source for merge of xl6 anon-WO0118547.78 was sequence comparison ( date:051113 ).

                  (FlyBase, 1996-)
                  Nomenclature History
                  Source for database identify of

                  Source for identity of: xl6 TN166

                  (Hirota, 2004.11.8)
                  Nomenclature comments
                  Etymology

                  Named after the mouse ortholog.

                  (Vorbruggen et al., 2000)
                  Synonyms and Secondary IDs (19)
                  Reported As
                  Symbol Synonym
                  2/30
                  (Roch et al., 1998)
                  9G8
                  (Truong et al., 2024, Weidman et al., 2022, Parniewska and Stocker, 2020, Tang et al., 2018, Pons et al., 2017, Sciabica and Hertel, 2006, Kumar and Lopez, 2005, Kumar and Lopez, 2004, Bourbon, 2000.2.9, Mount and Salz, 2000)
                  CG10203
                  (National Institute of Genetics Fly Stocks, 2022-, Kolkhof et al., 2017, Çiçek et al., 2016, Brooks et al., 2015, Ni et al., 2010.12.1, Ritson et al., 2010, Herold et al., 2009, Keleman et al., 2009.8.5, Grieder et al., 2007, Wagner et al., 2007, Qin et al., 2005, Allemand et al., 2001, Mount and Salz, 2000)
                  Dx16
                  (Vorbruggen et al., 2000)
                  Dxl6
                  (Xie et al., 2001, Vorbruggen et al., 2000, Vorbruggen et al., 2000, Vorbrüggen, 1999.9.14)
                  RBP1-like
                  (Price et al., 2000)
                  TN166
                  (Hirota, 2004.11.8, Hirota et al., 2003)
                  X16
                  (Ustaoglu et al., 2019, Ng et al., 2016, Kwong et al., 2015, He et al., 2014, Saccone et al., 2014)
                  X16/9G8
                  (Mount and Salz, 2000)
                  XL6
                  (Zhang et al., 2018, Bradley et al., 2015, Wagner et al., 2007)
                  Xl6
                  (Lasko, 2000)
                  anon-WO0118547.78
                  d9G8
                  (Gabut et al., 2007, Allemand et al., 2002, Allemand et al., 2001)
                  dx16
                  (Wan et al., 2008, Xu et al., 2008)
                  dxl6
                  (Wagner et al., 2007)
                  l(2)k00230
                  (Spradling et al., 1999, BDGP Project Members, 1994-1999)
                  x16
                  (Fingerhut et al., 2019, Gene Disruption Project members, 2018-, Clandinin and Owens, 2016-, Lee et al., 2016, Sarov et al., 2016, Brooks et al., 2015, Kwong et al., 2015, Zabidi et al., 2015, Olesnicky et al., 2014, Rumpf et al., 2014, Sopko et al., 2014, Kwon et al., 2013, Graveley et al., 2011, Ritson et al., 2010, Grieder et al., 2007)
                  xl6
                  (Dyson et al., 2025, Sen and Cox, 2016, Tikhonov et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Graveley et al., 2011, Ritson et al., 2010, Herold et al., 2009, Buszczak et al., 2007, Qin et al., 2005)
                  Name Synonyms
                  x16 splicing factor
                  xl6
                  Secondary FlyBase IDs
                  • FBgn0022271
                  • FBgn0040511
                  • FBgn0061658
                  Datasets (0)
                  Study focus (0)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  Study result (0)
                  Result
                  Result Type
                  Title
                  External Crossreferences and Linkouts ( 53 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                  UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                  Other crossreferences
                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                  BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  FlyMine - An integrated database for Drosophila genomics
                  InterPro - A database of protein families, domains and functional sites
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  Linkouts
                  Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                  DroID - A comprehensive database of gene and protein interactions.
                  DRSC - Results frm RNAi screens
                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                  Flygut - An atlas of the Drosophila adult midgut
                  FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                  KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                  References (98)