FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Nrx-1, CASK
FlyBase ID
FBig0000116300
Interaction Network
Interactions Browser links
Nrx-1 network
CASK network
Reported Interactions
FBrf0215389-1.coIP.WB
Reference
Sun et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-1 

DNRX

bait
CASK 

CAKI

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sun et al., 2009)
FBrf0215389-2.PD.WB
Reference
Sun et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CASK 

CAKI

bait

GST tag

CASK 
Nrx-1 

DNRX

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CASK 
PDZ domain
sufficient binding region
aa 504-576

coordinates relative to CASK-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Sun et al., 2009)
FBrf0215389-3.Y2H
Reference
Sun et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-1 

DNRX

bait

fused to GAL4 DNA-binding domain

Nrx-1 
Nrx-1 
CASK 

CAKI

prey

fused to GAL4 activation domain

CASK 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nrx-1 
C-terminal domain
sufficient binding region
aa 1716-1837

coordinates relative to Nrx-1-PA

Nrx-1 
C-terminal domain
necessary binding region
aa 1734-1837

coordinates relative to Nrx-1-PA

CASK 
PDZ domain
sufficient binding region
aa 504-576

coordinates relative to CASK-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sun et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Sun et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)