FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
spi, S
FlyBase ID
FBig0000116320
Interaction Network
Interactions Browser links
spi network
S network
Reported Interactions
FBrf0144878-1.coIP.WB
Reference
Tsruya et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
spi 

Spi

bait

FLAG tag

S 

Star

prey

HA tag

S 
S 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
S 
TMC domain
sufficient binding region
aa 1-322
S 
NTM domain
sufficient binding region
aa 259-598
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tsruya et al., 2002)
FBrf0144878-2.coIP.WB
Reference
Tsruya et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
S 

Star

bait

TAP or HA tag

spi 

Spi

prey

GFP or no tag

spi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spi 
EGF domain
necessary binding region
aa 78-117
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tsruya et al., 2002)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)