FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
E(z), escl
FlyBase ID
FBig0000116327
Interaction Network
Interactions Browser links
E(z) network
escl network
Reported Interactions
FBrf0201936-14.coIP.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait
escl 

ESCL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurzhals et al., 2008)
FBrf0201936-16.coIP.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
escl 

ESCL

bait
E(z) 

E(Z)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurzhals et al., 2008)
FBrf0201936-17.coIP.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait
escl 

ESCL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurzhals et al., 2008)
FBrf0201936-18.CH.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
escl 

ESCL

neutral component
escl 

ESCL

neutral component
escl 

ESCL

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; proteins produced from endogenous genes.

(Kurzhals et al., 2008)
FBrf0201936-2.PD.A
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait

GST tag

E(z) 
escl 

ESCL

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(z) 
N-terminal domain
sufficient binding region
aa 1-73
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kurzhals et al., 2008)
FBrf0201936-24.CH.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
escl 

ESCL

neutral component
escl 

ESCL

neutral component
escl 

ESCL

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurzhals et al., 2008)
FBrf0201936-30.CH.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
escl 

ESCL

neutral component
escl 

ESCL

neutral component
escl 

ESCL

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurzhals et al., 2008)
FBrf0201936-34.CH.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
E(z) 

E(Z)

neutral component
escl 

ESCL

neutral component
escl 

ESCL

neutral component
escl 

ESCL

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; proteins produced from endogenous genes.

(Kurzhals et al., 2008)
FBrf0201936-4.PD.A
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
escl 

ESCL

bait

GST tag

escl 
E(z) 

E(Z)

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
escl 
WD domain
sufficient binding region
aa 95-462
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kurzhals et al., 2008)
FBrf0201936-6.PD.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(Z)

bait

GST tag

E(z) 
escl 

ESCL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
E(z) 
N-terminal domain
sufficient binding region
aa 1-73
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from Kc cell extract.

(Kurzhals et al., 2008)
FBrf0201936-7.coIP.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
escl 

ESCL

bait
E(z) 

E(Z)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurzhals et al., 2008)
FBrf0201936-9.coIP.WB
Reference
Kurzhals et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
escl 

ESCL

bait
E(z) 

E(Z)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kurzhals et al., 2008)
FBrf0205395-3.coIP.MW
Reference
Ohno et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
E(z) 

E(z)

bait

FLAG tag

escl 

ESCL

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from baculovirus construct; prey produced from baculovirus construct.

(Ohno et al., 2008)

PRC2 complexes were reconstituted from cells infected with baculoviruses for E(z), Su(z)12, Caf1-55 and esc or escl.

(Ohno et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)