FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mad, nej
FlyBase ID
FBig0000116483
Interaction Network
Interactions Browser links
Mad network
nej network
Reported Interactions
FBrf0108296-1.Y2H
Reference
Waltzer and Bienz, 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

fused to LexA DNA-binding domain

Mad 
nej 

CBP

prey

fused to B42 activation domain

nej 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
interaction domain
sufficient binding region
aa 2240-2608
Mad 
MH2 domain
mutation decreasing interaction
aa 219-455
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD. Text and methods state Mad as bait and nej as prey, which is contradictory to the actual figure.

(Waltzer and Bienz, 1999)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Waltzer and Bienz, 1999)
FBrf0108296-2.PD.A
Reference
Waltzer and Bienz, 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

CBP

bait

GST tag

nej 
Mad 

Mad

prey

[35]S label

Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
interaction domain
sufficient binding region
aa 2240-2608
Mad 
MH2 domain
mutation decreasing interaction
aa 219-455
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Waltzer and Bienz, 1999)
FBrf0108296-3.PD.A
Reference
Waltzer and Bienz, 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

GST tag

Mad 
nej 

CBP

prey

[35]S label

nej 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
interaction domain
sufficient binding region
aa 2240-2608
Mad 
MH2 domain
mutation decreasing interaction
aa 219-455
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Waltzer and Bienz, 1999)
FBrf0192358-1.PD.A
Reference
Lilja et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

MAD

bait

GST tag

Mad 
nej 

dCBP

prey

[35]S label

nej 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
C-terminal domain
sufficient binding region
aa 2232-2545,2677-3190
Mad 
MH2 domain
sufficient binding region
aa 219-455
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lilja et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)