FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Dcr-2, wisp
FlyBase ID
FBig0000116525
Interaction Network
Interactions Browser links
Dcr-2 network
wisp network
Reported Interactions
FBrf0238454-10.coIP.WB
Reference
Coll et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wisp 

Wispy

bait
Dcr-2 

Dcr-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wild-type embryos; bait produced from endogenous gene; prey produced from endogenous gene.

(Coll et al., 2018)

Treatment with RNase had no effect on the wisp-Dcr-2 interaction.

(Coll et al., 2018)
FBrf0238454-11.coIP.WB
Reference
Coll et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dcr-2 

Dcr-2

bait
wisp 

Wispy

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Treatment with RNase had no effect on the wisp-Dcr-2 interaction.

(Coll et al., 2018)

Source was wild-type embryos; bait produced from endogenous gene; prey produced from endogenous gene.

(Coll et al., 2018)
FBrf0238454-12.coIP.A
Reference
Coll et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dcr-2 

Dcr-2

bait

His tag

wisp 

Wispy

prey

[35]S label

wisp 
wisp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
wisp 
C-terminal domain
sufficient binding region
aa 945-1373

coordinates relative to wisp-PA

wisp 
C-terminal domain
necessary binding region
aa 945-1373

coordinates relative to wisp-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 cell system; prey produced and labeled by in vitro translation.

(Coll et al., 2018)
FBrf0238454-13.coIP.WB
Reference
Coll et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wisp 

Wispy

bait
Dcr-2 

Ago-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wild-type embryos; bait produced from endogenous gene; prey produced from endogenous gene.

(Coll et al., 2018)
FBrf0254505-13.coIP.MS
Reference
Nadimpalli et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dcr-2 

Dcr-2

bait
wisp 

Wispy

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Dcr-2 null mutants used as a negative control.

(Nadimpalli et al., 2022)

Source was embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nadimpalli et al., 2022)
FBrf0254505-7.coIP.WB
Reference
Nadimpalli et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dcr-2 

Dcr-2

bait
wisp 

Wispy

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Nadimpalli et al., 2022)

Interaction RNA independent; interaction of Dcr-2 and wisp abrogated in Atx2 and tyf knock down embryos.

(Nadimpalli et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)