FlyBase Gene Report: Dmel\wisp

FB2025_05 , released December 11, 2025

FB2025_05 , released December 11, 2025

Gene: Dmel\wisp

General Information

Symbol

Dmel\wisp

Species

D. melanogaster

Name

wispy

Annotation Symbol

CG15737

Feature Type

protein_coding_gene

FlyBase ID

FBgn0260780

Gene Model Status

Stock Availability

8 publicly available

Enzyme Name (EC)

polynucleotide adenylyltransferase (2.7.7.19)

Gene Summary

wispy (wisp) encodes a conserved cytoplasmic poly-A polymerase of the GLD2 family that is expressed in the female germline and, at low levels, in the nervous system. Its maternal germline expression is essential for completion of normal meiosis by oocytes at egg activation. The product of wisp elongates the poly-A tails of many mRNAs during egg activation. This activity is essential for the translation of maternal mRNAs. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

fs(1)M19, TAMP

Key Links

Genomic Location

Cytogenetic map

Sequence location

X:11,893,943..11,899,920 [+]

Recombination map

1-37

RefSeq locus

NC_004354 REGION:11893943..11899920

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables poly(A) RNA polymerase activity

inferred from direct assay

(Benoit et al., 2008)

enables protein binding

inferred from physical interaction with UniProtKB:Q24433

(Benoit et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables poly(A) RNA polymerase activity

inferred from biological aspect of ancestor with PANTHER:PTN000957350

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q6PIY7

(Gene Ontology Curators, 2002-)

inferred from sequence or structural similarity with WB:WBGene00001596

(Wang et al., 2002)

Biological Process (13 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in cytosolic mRNA polyadenylation

inferred from mutant phenotype

(Benoit et al., 2008)

involved_in egg activation

inferred from mutant phenotype

(Cui et al., 2008, Tadros et al., 2003)

involved_in intracellular mRNA localization

inferred from mutant phenotype

(Brent et al., 2000)

involved_in mitotic spindle organization

inferred from mutant phenotype

(Brent et al., 2000)

involved_in mRNA 3'-end processing

inferred from mutant phenotype

(Cui et al., 2008)

involved_in oocyte maturation

inferred from mutant phenotype

(Cui et al., 2008)

involved_in oogenesis

inferred from mutant phenotype

(Benoit et al., 2008, Cui et al., 2008)

involved_in positive regulation of antibacterial peptide biosynthetic process

inferred from mutant phenotype

(Valanne et al., 2010)

involved_in pronuclear fusion

inferred from mutant phenotype

(Brent et al., 2000)

involved_in pronuclear migration

inferred from mutant phenotype

(Brent et al., 2000)

involved_in sperm aster formation

inferred from mutant phenotype

(Brent et al., 2000)

involved_in spindle assembly involved in female meiosis

inferred from mutant phenotype

(Brent et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in mRNA 3'-end processing

inferred from sequence or structural similarity with UniProtKB:Q6PIY7

(Gene Ontology Curators, 2002-)

involved_in RNA 3'-end processing

inferred from biological aspect of ancestor with PANTHER:PTN000263330

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Cui et al., 2008, Brent et al., 2000)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

POLY(A) POLYMERASES

Protein Family (UniProt)

Belongs to the DNA polymerase type-B-like family. GLD2 subfamily. (Q9VYS4)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

poly(A) RNA polymerase activity

RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate (2.7.7.19)

RHEA 11332:

Summaries

Gene Snapshot

wispy (wisp) encodes a conserved cytoplasmic poly-A polymerase of the GLD2 family that is expressed in the female germline and, at low levels, in the nervous system. Its maternal germline expression is essential for completion of normal meiosis by oocytes at egg activation. The product of wisp elongates the poly-A tails of many mRNAs during egg activation. This activity is essential for the translation of maternal mRNAs. [Date last reviewed: 2019-03-21]

Gene Group (FlyBase)

POLY(A) POLYMERASES -: Poly(A) polymerases (PAPs) catalyze the addition of nontemplated adenylyl residues to the 3' ends of RNA substrates. In eukaryotes, canonical PAPs polyadenylate mRNAs prior to export from the nucleus to the cytoplasm, which is required for the stabilization, export and efficient translation of transcripts. In contrast, non-canonical PAPs add poly(A) tails to a different types of RNAs to stimulate their degradation - similar to the function of bacterial poly(A) tails. PAPs therefore play crucial and diverse roles in the regulation of gene expression. (Adapted from PMID:21956911.)

Protein Function (UniProtKB)

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific maternal RNAs (bcd, Tl, and tor), forming a poly(A) tail, during late oogenesis and early embryogenesis (PubMed:18430932, PubMed:18434412, PubMed:29317541). In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs (PubMed:18430932, PubMed:18434412). Required for localization of mRNAs to both poles of the egg, to recruit or maintain known centrosomal proteins with two types of microtubule organizing centers (MTOCs): the central MTOC that forms between the meiosis II tandem spindles and the centrosomes of the mitotic spindle (PubMed:10747060). Required at the final stage of oogenesis for meiosis I metaphase arrest and for progression beyond this stage (PubMed:10747060, PubMed:12871909, PubMed:18434412). Functions with the RNA-binding protein Dcr-2 to promote cytoplasmic polyadenylation and translational activation of certain mRNAs such as Tl and r2d2 (PubMed:29317541). As a consequence, is involved in regulating Toll immune signaling and promoting resistance to fungal infection (PubMed:29317541).

(UniProt, Q9VYS4)

Summary (Interactive Fly)

poly(A) polymerase acting on specific mRNA targets during late oogenesis and early embryogenesis - required at the final stage of oogenesis for metaphase during meiosis I arrest - interacts with Orb genetically and physically in an ovarian complex.

Interactive Fly

Gene Model and Products

Number of Transcripts

3

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\wisp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VYS4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.51

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0073573

5127

1373

FBtr0342742

5124

1372

FBtr0342743

5320

1373

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0073417

151.3

1373

8.91

FBpp0309610

151.2

1372

8.86

FBpp0309611

151.3

1373

8.91

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1373 aa isoforms: wisp-PA, wisp-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with orb, an RNA-binding protein, generating an ovarian cytoplasmic polyadenylation complex (PubMed:18434412). Interacts (via C-terminus) with Dcr-2 (PubMed:29317541).

(UniProt, Q9VYS4)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\wisp using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

wisp_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.30

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

• pole plasm

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

(Fisher et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Cui et al., 2008)

adult stage | female

(Cui et al., 2008)

Additional Descriptive Data

A long form of wisp transcript detected by 3' primers is present in embryos and in adult females but not males. Primers containing sequences further 5' in the gene detect transcripts in males and females but the corresponding shorter transcript form was not characterized.

(Cui et al., 2008)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S5 -- S10B

• cytoplasm

(Benoit et al., 2008)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Cui et al., 2008)

embryonic stage 1 -- 4

(Benoit et al., 2008)

adult stage | female

(Cui et al., 2008)

(Cui et al., 2008)

(Cui et al., 2008)

Additional Descriptive Data

wisp protein is detected in ovarian oocytes.

(Cui et al., 2008)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Cui et al., 2008, Brent et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\wisp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\wisp

View exonic expression by tissue for Dmel\wisp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0260780

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32152/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260780

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030353

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260780

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15737

Flygut - An atlas of the Drosophila adult midgut

FBgn0260780

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 21 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of wisp

Transgenic constructs containing regulatory region of wisp

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene disrupted in

(Ryder, 2004.4.26)

(Tadros et al., 2003)

(Tadros et al., 2003, Brent et al., 2000)

(Benoit et al., 2008, Tadros et al., 2003, Brent et al., 2000)

Gene not disrupted in

(Tadros et al., 2003)

(Tadros et al., 2003, Brent et al., 2000)

Df(1)PoX2-1.688

(Kim et al., 2018)

Gene duplicated in

(Popodi et al., 2010-, Venken et al., 2010)

Inferred from location ( 25 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | maternal effect | recessive

lethal - all die during embryonic stage | maternal effect (with Df(1)RA47)

wisp^KG05287

lethal - all die during embryonic stage | maternal effect | recessive

lethal - all die during embryonic stage | non-rescuable maternal effect | recessive

wisp^KG05287

partially lethal - majority die, with Scer\GAL4^pnr-MD237

wisp^GD9101

wisp^KG05287

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

wisp^GD9101

viable, with Scer\GAL4^Mef2.PR

wisp^GD9101

viable, with Scer\GAL4^tin.CΔ4

wisp^GD9101

Sterility

Allele

female sterile | recessive

wisp^KG05287

Other Phenotypes

Allele

abnormal meiotic cell cycle | recessive

abnormal mitotic cell cycle | recessive

some die during embryonic stage | maternal effect (with Df(1)RA47)

wisp^KG05287

some die during embryonic stage | recessive

wisp^KG05287

some die during embryonic stage | recessive | maternal effect | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

wisp^GD9101

visible, with Scer\GAL4^pnr-MD237

wisp^GD9101

visible | larval stage, with Scer\GAL4^{VP16.mat.αTub67C}

wisp^UASp.cCa

Phenotype manifest in

Allele

egg | maternal effect

embryonic/larval cuticle | larval stage, with Scer\GAL4^{VP16.mat.αTub67C}

wisp^UASp.cCa

female pronucleus

larval abdominal segment, with Scer\GAL4^{VP16.mat.αTub67C}

wisp^UASp.cCa

meiosis & nuclear chromosome

meiotic cell cycle & aster

meiotic cell cycle & pericentriolar material

meiotic cell cycle & spindle

meiotic metaphase I

mesonotum, with Scer\GAL4^pnr-MD237

wisp^GD9101

mitosis & nuclear chromosome

mitotic cell cycle & centrosome

mitotic cell cycle & spindle

spindle | ectopic

trichogen cell, with Scer\GAL4^pnr-MD237

wisp^GD9101

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (7)

9 of 14

Yes

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1

2 of 14

No

No

1 of 14

No

No

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (7)

9 of 14

Yes

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Mus musculus (laboratory mouse) (7)

9 of 14

Yes

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

Xenopus tropicalis (Western clawed frog) (4)

6 of 13

Yes

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Danio rerio (Zebrafish) (7)

8 of 14

Yes

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

Caenorhabditis elegans (Nematode, roundworm) (16)

7 of 14

Yes

No

4 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Anopheles gambiae (African malaria mosquito) (7)

Agam\AgaP_AGAP001130

7 of 12

Yes

Yes

Agam\AgaP_AGAP003314

4 of 12

No

Yes

Agam\AgaP_AGAP006659

3 of 12

No

No

Agam\AgaP_AGAP001564

2 of 12

No

No

Agam\AgaP_AGAP003293

2 of 12

No

No

Agam\AgaP_AGAP003771

2 of 12

No

No

Agam\AgaP_AGAP013038

2 of 12

No

No

Arabidopsis thaliana (thale-cress) (11)

5 of 13

Yes

Yes

4 of 13

No

Yes

3 of 13

No

No

3 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

Yes

2 of 13

No

No

2 of 13

No

No

1 of 13

No

No

Saccharomyces cerevisiae (Brewer's yeast) (2)

2 of 13

Yes

No

2 of 13

Yes

No

Schizosaccharomyces pombe (Fission yeast) (6)

5 of 12

Yes

Yes

5 of 12

Yes

Yes

5 of 12

Yes

Yes

3 of 12

No

Yes

2 of 12

No

No

2 of 12

No

No

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:wisp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

10 of 13

4 of 13

4 of 13

4 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TENT2; terminal nucleotidyltransferase 2

9 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

affinity chromatography technology, Identification by mass spectrometry, anti bait coimmunoprecipitation, primer specific pcr, western blot, quantitative reverse transcription pcr

(Coll et al., 2018, Cui et al., 2013)

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

enzymatic study, autoradiography

(Lee et al., 2014)

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

anti bait coimmunoprecipitation, primer specific pcr

(Cui et al., 2013)

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot

(Lee et al., 2014)

anti bait coimmunoprecipitation, western blot

(Nadimpalli et al., 2022)

two hybrid, anti bait coimmunoprecipitation, western blot

(Benoit et al., 2008, Cui et al., 2008)

anti bait coimmunoprecipitation, autoradiography, western blot, Identification by mass spectrometry

(Nadimpalli et al., 2022, Coll et al., 2018)

anti tag coimmunoprecipitation, western blot, pull down, anti tag western blot, anti bait coimmunoprecipitation

(Dufourt et al., 2017)

anti bait coimmunoprecipitation, western blot

(Benoit et al., 2008)

pull down, western blot, anti bait coimmunoprecipitation

(Wong et al., 2011, Benoit et al., 2008)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Benoit et al., 2008)

External Data

Subunit Structure (UniProtKB)

Interacts with orb, an RNA-binding protein, generating an ovarian cytoplasmic polyadenylation complex (PubMed:18434412). Interacts (via C-terminus) with Dcr-2 (PubMed:29317541).

(UniProt, Q9VYS4 )

Linkouts

BioGRID - A database of protein and genetic interactions.

58557

DroID - A comprehensive database of gene and protein interactions.

FBgn0260780

MIST (protein-protein) - An integrated Molecular Interaction Database

32152

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

X

Recombination map

1-37

Cytogenetic map

Sequence location

X:11,893,943..11,899,920 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

10F4-10F4

Limits computationally determined from genome sequence between P{EP}ATP7^EP308 and P{EP}EP1451&P{EP}EP1629

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

11A1-11A2

(determined by in situ hybridisation)

(Peter et al., 2002)

Experimentally Determined Recombination Data

Location

1-37

(FlyBase, 2016)

1-36.8

(Comeron et al., 2012)

1-

(Mohler, 1977)

Left of (cM)

f

(Mohler, 1977)

Right of (cM)

v

(Mohler, 1977)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

607150

w^*; P{UAS-wisp.W}3

43141

y¹ v¹; P{TRiP.GL01482}attP2

16467

y¹ P{SUPor-P}wisp^KG05287/FM7c

607137

y¹ w¹¹¹⁸ wisp^14-1299/FM6

4613

y¹ cv¹ v¹ wisp^12-3147 f¹/FM0

123961

w¹¹¹⁸ P{RS3}wisp^CB-6172-3

v105972

P{KK101448}VIE-260B

v19584

w¹¹¹⁸; P{GD9101}v19584

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (47)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE03648

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0260780

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0260780

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lee et al., 2014, Laflamme et al., 2012, Benoit et al., 2008, Cui et al., 2008)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

sub mutants do not interact with alleles of γTub37C, αTub67C, msps, tacc or wisp.

(Giunta et al., 2002)

wisp is required to recruit or maintain known centrosomal proteins with two types of microtubule organising centers (MTOCs); the central MTOC that forms between the meiosis II tandem spindles and the centrosomes of the mitotic spindle.

(Brent et al., 2000)

Both the wisp and aim mutant phenotypes suggest that these genes play important structural or regulatory roles in functions performed by microtubules.

(Hazelrigg et al., 1998)

Maternal effect alleles cause embryos to arrest immediately following meiosis. Meiotic defects include perturbations of metaphase I arrest, and chromosome non-disjunction and loss. The astral array of microtubules between the two meiosis II spindles is small or missing, and lacks normal levels of gamma-tubulin and other centrosomal proteins normally present in this structure. After fertilization, long, wispy meiotic-like spindles form on the products of female meiosis. These embryos arrest with a small spindle formed on the haploid male pronucleus, as centrosomes of this spindle are lost in the nearby cytoplasm.

(Hazelrigg et al., 1998)

Relationship to Other Genes

Source for database merge of

Source for merge of: wisp fs(1)M19

(Brent et al., 2000)

Source for merge of: wisp CG15737

(Benoit et al., 2008, Cui et al., 2008)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

The gene is named "temprano", the Spanish word for "early", as mutant embryos show defects in the early syncytial stages.

(Tadros et al., 2003)

The gene is named "wispy" because of its mutant effects on spindle structures.

(Brent et al., 2000)

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG15737

(Shan et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, Ni et al., 2010.12.1, Valanne et al., 2010, Keleman et al., 2009.8.5, Benoit et al., 2008, Cui et al., 2008, Brandt, 2006)

DmCG15737

(Wang et al., 2002)

PAP

(Jia Ng et al., 2018)

TAMP

(Valanne, 2014, Valanne et al., 2010)

TUTase2

(Modepalli and Moran, 2017)

Wisp

(Lee et al., 2025, Dufourt et al., 2017, Shan et al., 2017, Laflamme et al., 2012, Wong et al., 2011)

Wispy

(Schneider and Imler, 2021, Hughes et al., 2018, Rojas-Ríos and Simonelig, 2018)

fs(1)M19

(Mohler and Carroll, 1984, Mohler, 1977)

wisp

(Pérez-Roldán et al., 2025, Huang et al., 2024, Curnutte et al., 2023, Gupta et al., 2022, Wei et al., 2018, Lim et al., 2016, Hsu et al., 2015, Lee et al., 2014, Cui et al., 2013, Kwon et al., 2013, Webber et al., 2013, Popodi et al., 2010-, Thomsen et al., 2010, Venken et al., 2010, Benoit et al., 2008, Cui et al., 2008, Cui et al., 2008)

wispy

(Chartier et al., 2015, Valanne, 2014)

Name Synonyms

3' Terminal Uridylyl Transferase 2

(Modepalli and Moran, 2017)

CG15737

Toll pathway activation mediating protein

(Valanne et al., 2010)

WISPY

(Cui et al., 2013)

Wispy

(Patel et al., 2024, Wang and Lin, 2021, Dufourt et al., 2017, Modepalli and Moran, 2017)

female sterile(1)M19

poly(A) polymerase

(Jia Ng et al., 2018)

temprano

(Tadros et al., 2003)

wispy

(Jia Ng et al., 2018, Derrick and Weil, 2017, Eichhorn et al., 2016, Lim et al., 2016, Benoit et al., 2008, Cui et al., 2008, Cui et al., 2008, Hazelrigg et al., 1998)

Secondary FlyBase IDs

FBgn0024180
FBgn0000835
FBgn0030353

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32152

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VYS4

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VYS4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VYS4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0260780

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32152/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260780

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260780

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15737

MARRVEL_MODEL - MARRVEL (model organism gene)

32152

Linkouts

BioGRID - A database of protein and genetic interactions.

58557

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0260780

DroID - A comprehensive database of gene and protein interactions.

FBgn0260780

DRSC - Results frm RNAi screens

FBgn0260780

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

wisp_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030353

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0260780

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15737

Flygut - An atlas of the Drosophila adult midgut

FBgn0260780

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

32152

References (80)