Ras1
GST tag
Dp110
Myc tag
dominant negative Ras1 N17
T231D, K250A, R253A and K257A
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell culture extract.
The Ras85D-Pi3K92E interaction was detected with the constitutively active V12 form of Ras.
fused to GAL4 DNA-binding domain
fused to VP16 activation domain
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/VP16-AD, The Ras85D-Pi3K92E interaction was detected with the constitutively active V12 form of Ras.
