FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pits, Sin3A
FlyBase ID
FBig0000116594
Interaction Network
Interactions Browser links
Pits network
Sin3A network
Reported Interactions
FBrf0238907-3.PD.WB
Reference
Liaw, 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pits 

CG11138

bait

GST tag

Pits 
Pits 
Sin3A 

Sin3A

prey

S tag

Sin3A 
Sin3A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Pits 
N-terminal domain
sufficient binding region
aa 2-95

coordinates relative to Pits-PD, binds to PAH3 fragment of Sin3A, aa 918-1352.

Pits 
middle domain
sufficient binding region
aa 160-406

coordinates relative to Pits-PD, binds to PAH1 fragment of Sin3A, aa 3-371.

Sin3A 
PAH1 domain
sufficient binding region
aa 3-371

coordinates relative to Sin3A-PE, binds to full length Pits and the middle fragment of Pits, aa 160-406.

Sin3A 
PAH3 domain
sufficient binding region
aa 918-1352

coordinates relative to Sin3A-PE, binds to N-terminal fragment of Pits, aa 2-95.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Liaw, 2016)
FBrf0238907-5.coIP.WB
Reference
Liaw, 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pits 

CG11138

bait
Sin3A 

Sin3A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Liaw, 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)