FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
14-3-3ε, Hsap\MAPT
FlyBase ID
FBig0000116642
Interaction Network
Interactions Browser links
14-3-3ε network
Hsap\MAPT network
Reported Interactions
FBrf0239248-2.coIP.WB
Reference
Papanikolopoulou et al., 2018
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hsap\MAPT 

TAU

bait

FLAG tag

14-3-3ε 

EPS

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Papanikolopoulou et al., 2018)
FBrf0239248-4.AF.WB
Reference
Papanikolopoulou et al., 2018
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
14-3-3ε 

EPS

bait
Hsap\MAPT 

TAU

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GST-difopein-conjugated beads were used to pull down 14-3-3 proteins from fly head lysates. Co-purifying Hsap\MAPT was detected by western blot.

(Papanikolopoulou et al., 2018)

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.

(Papanikolopoulou et al., 2018)
FBrf0253129-5.coIP.MS
Reference
Prifti et al., 2022
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hsap\MAPT 

htau

bait

Flag tag

14-3-3ε 

14-3-3ε

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control. Interaction increased upon over-expression of Mical.

(Prifti et al., 2022)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Prifti et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (2)