FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mle, Polr2A
FlyBase ID
FBig0000116752
Interaction Network
Interactions Browser links
mle network
Polr2A network
Reported Interactions
FBrf0204293-1.PD.WB
Reference
Aratani et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mle 

MLE

bait

GST tag

mle 
mle 
mle 
Polr2A 

PolII

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mle 
MLE2 domain
sufficient binding region
aa 219-653
mle 
MTAD domain
necessary binding region
aa 326-378
mle 
MLE2 domain residues 328,335,338
mutation disrupting interaction
aa 328,335,338

W328A,W335A,W338A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell nuclear extract.

(Aratani et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)