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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116798
Interaction Network
Interactions Browser links
p53 network
Taf3 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0205137-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait

fused to LexA DNA-binding domain

Taf3 

DmTAF3

prey

fused to B42 activation domain

Taf3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf3 
interaction domain
sufficient binding region
aa 514-924aa 738-1061
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/B42-AD

FBrf0205137-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait

fused to LexA DNA-binding domain

p53 
Taf3 

DmTAF3

prey

fused to B42 activation domain

Taf3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Taf3 
interaction domain
sufficient binding region
aa 514-924
p53 
C-terminal domain
sufficient binding region
aa 277-351

coordinates relative to p53-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

FBrf0205137-3.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p53 

Dmp53

bait

GST tag

p53 
Taf3 

DmTAF3

prey

[3]H label

Taf3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
p53 
C-terminal domain
sufficient binding region
aa 277-385

coordinates relative to p53-PA

Taf3 
interaction domain
sufficient binding region
aa 514-924
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

External Crossreferences and Linkouts ( 0 )
References (1)