FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hth, abd-A
FlyBase ID
FBig0000117155
Interaction Network
Interactions Browser links
hth network
abd-A network
Reported Interactions
FBrf0179749-2.EMSA.A
Reference
Gebelein et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

hth

neutral component

His tag

hth 

hth

neutral component

His tag

hth 

hth

neutral component

His tag

abd-A 

abd-A

neutral component

His tag

abd-A 

abd-A

neutral component

His tag

abd-A 

abd-A

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

exd and hth were expressed and purified together as a heterodimer. Addition of abd-A results in trimer and tetramer formation on [32]P labeled DNA. Formation of these higher order complexes is

(Gebelein et al., 2004)

inhibited and partially supershifted by the addition of anti-En antibody indicating the complex contains en.

(Gebelein et al., 2004)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Gebelein et al., 2004)
FBrf0240351-132.BiFC.FM
Reference
Bischof et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hth 

Hth

bait

YFP tag, N-terminal fragment

abd-A 

AbdA

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

(Bischof et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (2)