FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Lasp, Actin
FlyBase ID
FBig0000117344
Interaction Network
Interactions Browser links
Lasp network
Actin network
Reported Interactions
FBrf0225918-18a.TAP.WB
Reference
Fernandes and Schöck, 2014
Description
physical association
Assay
Collection
Source/Stage
adult thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lasp 

Lasp

bait

FLAG tag, His tag

Lasp 
Actin 

Actin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Lasp 
nebulin repeats
necessary binding region
aa 106-171

coordinates relative to Lasp-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thoraces of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fernandes and Schöck, 2014)

Deletion of the Lasp Lim domain or SH3 domain, or mutations Y86S or Y121S do not interfere with Actin binding.

(Fernandes and Schöck, 2014)
FBrf0225918-6a.coIP.MS
Reference
Fernandes and Schöck, 2014
Description
physical association
Assay
Collection
Source/Stage
adult thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Lasp 

Lasp

bait

GFP tag

Actin 

Actin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thoraces of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fernandes and Schöck, 2014)

Actin was enriched in Lasp-GFP coimmunoprecipitation compared to GFP-only coimmunoprecipitation.

(Fernandes and Schöck, 2014)
External Crossreferences and Linkouts ( 0 )
References (1)