Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The Pu-ple interaction was shown with Pu isoforms B and C which correspond to Pu-PC and Pu-PA.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The Pu-ple interaction was shown with Pu isoforms B and C which correspond to Pu-PC and Pu-PA.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The Pu-ple interaction was shown with Pu isoforms B and C which correspond to Pu-PC and Pu-PA. Pu isoform B interacts with ple regardless of phosphorylation state, while isoform C interacts with ple only when both proteins are phosphorylated.

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Treatment of head extracts with alkaline phosphatase disrupts the interaction between ple and Pu isoform C.