FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mad1, Mgtor
FlyBase ID
FBig0000117530
Interaction Network
Interactions Browser links
Mad1 network
Mgtor network
Reported Interactions
FBrf0240398-2.coIP.MS
Reference
Raich et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad1 

Mad1

bait

GFP tag

Mgtor 

Mtor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Raich et al., 2018)
FBrf0240398-6.coIP.WB
Reference
Raich et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad1 

Mad1

bait

GFP tag

Mgtor 

Mtor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Raich et al., 2018)
FBrf0240398-8.coIP.WB
Reference
Raich et al., 2018
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad1 

Mad1

bait

GFP tag

Mgtor 

Mtor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testis of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Raich et al., 2018)
FBrf0244511-1.coIP.WB
Reference
Cunha-Silva et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Mad1 

Mad1

bait
Mgtor 

Mtor

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Cunha-Silva et al., 2020)
FBrf0244511-2.PD.WB
Reference
Cunha-Silva et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad1 

Mad1

bait

His tag

Mad1 
Mgtor 

Mtor

prey

MBP tag

Mgtor 
Mgtor 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad1 
N-terminal domain
sufficient binding region
aa 1-493
Mgtor 
coiled-coil domain
sufficient binding region
aa 1-402aa 403-800aa 1187-1655
Mgtor 
coiled-coil domain residues 1259,1338,1390
mutation disrupting interaction
aa 1259,1338,1390

T1259D,T1338D,T1390D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Cunha-Silva et al., 2020)

Treatment of Mtor with Mps1 disrupts its interaction with Mad1.

(Cunha-Silva et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (2)