FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
HP1b, Polr2A
FlyBase ID
FBig0000117578
Interaction Network
Interactions Browser links
HP1b network
Polr2A network
Reported Interactions
FBrf0241416-37.coIP.WB
Reference
Lee et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
HP1b 

HP1b

bait

FLAG tag

Polr2A 

PolII

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.

(Lee et al., 2019)
FBrf0241416-40.PD.WB
Reference
Lee et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Polr2A 

PolII

bait

biotin label

HP1b 

HP1b

prey

FLAG tag

HP1b 
HP1b 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
HP1b 
Chromo domain
sufficient binding region
aa 1-92
HP1b 
Chromo domain
necessary binding region
aa 1-55
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide chemically synthesized and labeled in vitro; prey produced as a recombinant fusion protein in baculovirus and Sf21 cell system.

(Lee et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)