FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
DIP-α
FlyBase ID
FBig0000117653
Interaction Network
Interactions Browser links
DIP-α network
Reported Interactions
FBrf0241075-4.CS.MW
Reference
Cosmanescu et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-α 

DIP-α

neutral component
DIP-α 

DIP-α

neutral component
DIP-α 
DIP-α 
DIP-α 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DIP-α 
extracellular domain
sufficient binding region
aa 40-341

coordinates relative to DIP-α-PA

DIP-α 
extracellular domain residue 83
mutation disrupting interaction
aa 83

I83D coordinates relative to DIP-α-PA

DIP-α 
extracellular domain residues 78,94
mutation disrupting interaction
aa 78,94

A78K,N94D coordinates relative to DIP-α-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in HEK293F cells.

(Cosmanescu et al., 2018)

behaves as a dimer

(Cosmanescu et al., 2018)
FBrf0241075-58.XC
Reference
Cosmanescu et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-α 

DIP-α

neutral component
DIP-α 

DIP-α

neutral component
DIP-α 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DIP-α 
extracellular domain
sufficient binding region
aa 40-341

coordinates relative to DIP-α-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in HEK293F cells.

(Cosmanescu et al., 2018)
FBrf0241500-11.CH.MW
Reference
Cheng et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-α 

DIP-α

neutral component
DIP-α 

DIP-α

neutral component
DIP-α 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DIP-α 
IG1 domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculoviral Sf9 cell system.

(Cheng et al., 2019)

DIP-α forms a homodimer in solution. Kd = 37uM.

(Cheng et al., 2019)
FBrf0241500-13.ELISA
Reference
Cheng et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
DIP-α 

DIP-α

bait

Fc tag

DIP-α 

DIP-α

prey

AP tag

DIP-α 
DIP-α 
DIP-α 
DIP-α 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DIP-α 
IG1 domain
sufficient binding region
unspecified
DIP-α 
IG1 domain residue 83
mutation disrupting interaction
aa 83

I83A

DIP-α 
IG1 domain residue 91
mutation decreasing interaction
aa 91

I91A

DIP-α 
IG1 domain residue 125
mutation decreasing interaction
aa 83

Q125A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned medium of transfected S2 cell line; prey derived from conditioned medium of transfected S2 cell line.

(Cheng et al., 2019)
FBrf0245556-5.CS.MW
Reference
Sergeeva et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-α 

AA

neutral component
DIP-α 

AA

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein purified from transfected HEK293F cells.

(Sergeeva et al., 2020)
FBrf0253099-3.SPR
Reference
Xu et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DIP-α 

DIP-α

bait

His tag

DIP-α 

DIP-α

prey

His tag

DIP-α 
DIP-α 
DIP-α 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DIP-α 
interaction domain residue 74
mutation decreasing interaction
aa 74

G74A

DIP-α 
interaction domain residue 81
mutation increasing interaction
aa 81

K81Q

DIP-α 
interaction domain residues 81 and 74
mutation decreasing interaction
aa 81 and 74

K81Q, G74A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced from transfected constructs in HEK293F cells.

(Xu et al., 2022)

Wild type and mutant DIP-α homodimer Kds: wild type 23.9, G74A 50, K81Q 19.7, K81Q and G74S 46.3.

(Xu et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (4)