FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
shep, brm
FlyBase ID
FBig0000118032
Interaction Network
Interactions Browser links
shep network
brm network
Reported Interactions
FBrf0241708-1.EMSA.A
Reference
Chen et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shep 

Shep E

bait

His tag

shep 
brm 

brm

prey

[32]P label

brm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brm 
shep-binding site
sufficient binding region
nt 4831-5282

coordinates relative to brm-RA

shep 
RRM domain residues 64,66,69,162,164,166
mutation disrupting interaction
aa 64,66,69,162,164,166

Y64A,F66A,F69A,V162A,F164A,R166A, coordinates relative to shep-PE

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Chen et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)