FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
grk, D
FlyBase ID
FBig0000119767
Interaction Network
Interactions Browser links
grk network
D network
Reported Interactions
FBrf0192288-1.Y3H
Reference
Mukherjee et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
grk 

gurken

bait

fused to MS2-binding domain

grk 
D 

D

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grk 
3-prime UTR
sufficient binding region
nt 1269-1699

coordinates relative to grk-RA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced from transgenic construct; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Mukherjee et al., 2006)

Three-hybrid system: DNA binding component is lexA fused to MS2 RNA binding protein, bridging component is bait mRNA in tandem to MS2 binding sites, transcriptional activation component is prey protein fused to B42.

(Mukherjee et al., 2006)
FBrf0192288-2.coIP.PCR
Reference
Mukherjee et al., 2006
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
D 

D

bait
grk 

gurken

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Mukherjee et al., 2006)
External Crossreferences and Linkouts ( 0 )
References (1)