FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Arf6, pav
FlyBase ID
FBig0000119773
Interaction Network
Interactions Browser links
Arf6 network
pav network
Reported Interactions
FBrf0202075-1.Y2H
Reference
Dyer et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arf6 

ARF6

bait

Q67L mutant form of Arf51F,fused to LexA DNA-binding domain

pav 

Pav

prey

fused to GAL4 activation domain

pav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pav 
interaction domain
sufficient binding region
aa 727-860
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Dyer et al., 2007)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Dyer et al., 2007)
FBrf0202075-2.PD.WB
Reference
Dyer et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arf6 

ARF6

bait

GST tag

Arf6 
pav 

Pav

prey

HA tag

pav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pav 
interaction domain
sufficient binding region
aa 655-865
Arf6 
interaction domain residue 67
mutation increasing interaction
aa 67

Q67L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HeLa cell extract.

(Dyer et al., 2007)
External Crossreferences and Linkouts ( 0 )
References (1)