FlyBase Gene Report: Dmel\Arf6

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anterior midgut primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

garland cell primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic/larval plasmatocyte

(Fisher et al., 2012)

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

head

(Lee et al., 1994)

Additional Descriptive Data

Arf6 transcripts are significantly more abundant in heads than in other adult body parts

(Lee et al., 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with adherens junction

inferred from direct assay

(Johnson et al., 2011)

located_in cleavage furrow

inferred from direct assay

(Lee et al., 2015)

located_in cytosol

inferred from direct assay

(Dyer et al., 2007)

located_in early endosome

inferred from direct assay

(Dyer et al., 2007)

located_in plasma membrane

inferred from direct assay

(Dyer et al., 2007)

located_in recycling endosome

inferred from direct assay

(Dyer et al., 2007)

Expression Deduced from Reporters

Reporter: P{GawB}Arf6^NP5226

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult mushroom body

(Peru Y Colón de Portugal et al., 2012)

adult insulin secreting cell of pars intercerebralis

(Peru Y Colón de Portugal et al., 2012)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Arf6 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Arf6

View exonic expression by tissue for Dmel\Arf6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013750

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36699/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013750

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013750

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013750

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8156

Flygut - An atlas of the Drosophila adult midgut

FBgn0013750

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Arf6

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Arf6

Transgenic constructs containing regulatory region of Arf6

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Scer\GAL4^Mef2.PR

Arf6^GD13822

viable, with Scer\GAL4^pnr-MD237

Arf6^GD13822

viable, with Scer\GAL4^tin.CΔ4

Arf6^GD13822

Sterility

Allele

female semi-sterile

Arf6¹

male sterile

Arf6¹

Other Phenotypes

Allele

abnormal cell shape, with Scer\GAL4^en-e16E

Arf6^UAS.cCa

abnormal cytokinesis | male

Arf6¹

abnormal feeding behavior | adult stage | conditional

Arf6^EP2612

chemical resistant, with Scer\GAL4^elav-C155

Arf6^UAS.cCa

chemical sensitive (with Arf6^EP2612)

chemical sensitive (with Arf6^KG02753)

Arf6^EP2612

chemical sensitive (with Arf6^NP5226)

Arf6^EP2612

chemical sensitive | adult stage

chemical sensitive | adult stage (with Arf6^EP2612)

Arf6^NP5226

chemical sensitive | adult stage (with Arf6^NP5226)

Arf6^EP2612

chemical sensitive | recessive

Arf6^EP2612

Phenotype manifest in

Allele

cleavage furrow & primary spermatocyte

Arf6¹

embryonic myoblast, with Scer\GAL4^{kirre-rP298-G4}

Arf6^T27N.UAS

larval ventral nerve cord commissure | embryonic stage, with Scer\GAL4^rho.PL

Arf6^T27N.UAS

mechanosensory medial triple row bristle | decreased number

nucleus | embryonic stage | maternal effect

Arf6^ΔKG1

primary spermatocyte

retina, with Scer\GAL4^GMR.PF

Arf6^T27N.UAS

spermatid

Arf6¹

wing margin bristle | decreased number

wing margin bristle | decreased number (with Arf6¹)

Arf6^GX16w-

wing margin bristle | decreased number (with Arf6^GX16w-)

Arf6¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (48)

Hsap\ARF6

14 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

Hsap\ARL14

3 of 14

Hsap\ARL15

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (29)

Rnor\Arf6

14 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

Rnor\Arl14

3 of 14

Rnor\Arl15

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Arl9

1 of 14

Rnor\Arl10

1 of 14

Rnor\Arl13a

1 of 14

Yes

Rnor\Arl13b

1 of 14

Rnor\Arl16

1 of 14

Rnor\Sar1b

1 of 14

Mus musculus (laboratory mouse) (30)

Mmus\Arf6

14 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

Mmus\Arf5

3 of 14

Mmus\Arl14

3 of 14

Mmus\Arl15

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (36)

Xtro\arf6.2

7 of 13

Yes

Xtro\arf6

5 of 13

Yes

Xtro\arf5

4 of 13

Xtro\arl11

4 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\arl13a

1 of 13

Yes

Xtro\arl13b

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (44)

Drer\arf6a

13 of 14

Yes

Drer\arf6b

13 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

Drer\zgc:77650

4 of 14

Drer\arl14

3 of 14

Drer\arl15a

3 of 14

Yes

Drer\arl15b

3 of 14

Yes

Drer\si:ch1073-165f9.2

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (34)

Cele\arf-6

13 of 14

Yes

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (18)

Agam\AgaP_AGAP006890

11 of 12

Yes

4 of 12

4 of 12

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (27)

Atha\ARF1

4 of 13

Yes

Atha\ARFA1D

4 of 13

Yes

Atha\ARFA1E

4 of 13

Yes

Atha\ARFA1F

4 of 13

Yes

Atha\ARFB1A

4 of 13

Yes

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (9)

Scer\ARF3

8 of 13

Yes

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\arf6

12 of 12

Yes

Spom\arf1

3 of 12

Spom\alp41

1 of 12

Spom\sar1

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Arf6. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (21)

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

dnd

2 of 13

Sar1

2 of 13

CG11356

1 of 13

CG17760

1 of 13

cta

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

Arf6^EP2612

model of alcohol use disorder

CEA

(Gonzalez et al., 2018)

CEA with Arf6^NP5226

(Gonzalez et al., 2018)

Arf6^KK108126

model of candidiasis

CEA

(Glittenberg et al., 2022)

Arf6^NP5226

model of alcohol use disorder

CEA with Arf6^EP2612

(Gonzalez et al., 2018)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

14 of 14

4 of 14

PERIVENTRICULAR NODULAR HETEROTOPIA 8; PVNH8

4 of 14

4 of 14

4 of 14

ARL11; ARF like GTPase 11

4 of 14

609351

ARL14; ARF like GTPase 14

3 of 14

614439

ARL15; ARF like GTPase 15

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Arf6 - Arfip

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Chang et al., 2013, Peru Y Colón de Portugal et al., 2012)

Arf6 - Iqsec

enzymatic study, autoradiography

(Chen et al., 2003)

Arf6 - hh

anti bait coimmunoprecipitation, western blot

(Chabu et al., 2017)

Arf6 - pav

two hybrid, pull down, western blot

(Dyer et al., 2007)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

amn

(Peru Y Colón de Portugal et al., 2012)

enhanceable

Arf1

(Marcetteau et al., 2021)

enhanceable

ArfGAP3

(Johnson et al., 2011)

enhanceable

arm

(Marcetteau et al., 2021)

Dcr-2|Arf6

enhanceable

Asap

(Johnson et al., 2011)

enhanceable

dsh

(Marcetteau et al., 2021)

Dcr-2|Arf6

enhanceable

Efa6

(Johnson et al., 2011)

enhanceable

hbs

(Johnson et al., 2011)

enhanceable

Iqsec

(Johnson et al., 2011)

suppressible

pav

(Marcetteau et al., 2021)

enhanceable

Rho1

(Johnson et al., 2011)

enhanceable

(Marcetteau et al., 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

arm

suppressible

(Marcetteau et al., 2021)

Axn

suppressible

(Marcetteau et al., 2021)

cindr

enhanceable

(Johnson et al., 2011)

conu

enhanceable

(Neisch et al., 2013)

dsh

suppressible

(Marcetteau et al., 2021)

Rac1

enhanceable

(Neisch et al., 2013)

RhoGAP18B

suppressible

(Gonzalez et al., 2018, Peru Y Colón de Portugal et al., 2012)

sens

enhanceable

(Marcetteau et al., 2021)

sgg

suppressible