FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Arfip, Arf6
FlyBase ID
FBig0000097004
Interaction Network
Interactions Browser links
Arfip network
Arf6 network
Reported Interactions
FBrf0220158-2.coIP.WB
Reference
Peru Y Colón de Portugal et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Arfip 

Arfip

bait

FLAG tag

Arf6 

Arf6

prey

YFP tag

Arf6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arf6 
region
mutation decreasing interaction
aa 44

T44N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Peru Y Colón de Portugal et al., 2012)

Arfip binds to GTP-bound Arf51F, as inferred by its strong interaction with the Arf51F Q67L mutant (mimics GTP-locked state) but only weak binding to the Arf51F T44N mutant (mimics the GDP-bound state).

(Peru Y Colón de Portugal et al., 2012)
FBrf0220158-3.coIP.WB
Reference
Peru Y Colón de Portugal et al., 2012
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arfip 

Arfip

bait

HA tag

Arf6 

Arf6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Peru Y Colón de Portugal et al., 2012)

Arfip interacts with Rac1 and Arf51F, but not Cdc42.

(Peru Y Colón de Portugal et al., 2012)
FBrf0221747-3.coIP.WB
Reference
Chang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Arfip 

Arfip

bait

FLAG tag

Arf6 

Arf6

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of key Arginine residues (aa 243-256) in the BAR domain of Arfip does not affect its interaction with Arf51F (Arf6).

(Chang et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chang et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)