FlyBase Gene Report: Dmel\Efa6

General Information

Symbol

Dmel\Efa6

Species

D. melanogaster

Name

Exchange factor for Arf 6

Annotation Symbol

CG31158

Feature Type

protein_coding_gene

FlyBase ID

FBgn0051158

Gene Model Status

Current

Stock Availability

17 publicly available

Gene Summary

Exchange factor for Arf 6 (Efa6) encodes a Guanine Nucleotide Exchange Factor (GEF) that sustains activation of Arf family GTPases. They are active in dynamic actin rearrangement during cell shapes changes as well as cell patterning within epithelia. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

94B5-94B6

Sequence location

3R:22,587,755..22,608,842 [+]

Recombination map

3-75

RefSeq locus

NT_033777 REGION:22587755..22608842

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables microtubule binding

inferred from direct assay

(Qu et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables guanyl-nucleotide exchange factor activity

inferred from sequence or structural similarity with UniProtKB:Q06836

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR000904, InterPro:IPR035999

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN008950430

(GO Reference Genome Project, 2011-)

enables phospholipid binding

inferred from electronic annotation with InterPro:IPR001605

(InterPro Project Members, 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in axoneme assembly

inferred from mutant phenotype

(Qu et al., 2019)

involved_in compound eye morphogenesis

inferred from genetic interaction with UniProtKB:P40946

(Johnson et al., 2011)

inferred from genetic interaction with UniProtKB:Q9Y154

(Johnson et al., 2011)

involved_in negative regulation of microtubule polymerization

inferred from mutant phenotype

(Qu et al., 2019)

involved_in response to alcohol

inferred from genetic interaction with UniProtKB:P40946

(Gonzalez et al., 2018)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in positive regulation of ARF protein signal transduction

inferred from sequence or structural similarity with UniProtKB:Q06836

(Gene Ontology Curators, 2002-)

involved_in regulation of ARF protein signal transduction

inferred from electronic annotation with InterPro:IPR000904, InterPro:IPR035999

(InterPro Project Members, 2004-)

involved_in vesicle-mediated transport

inferred from biological aspect of ancestor with PANTHER:PTN008950430

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

colocalizes_with adherens junction

inferred from direct assay

(Johnson et al., 2011)

located_in axon

inferred from direct assay

(Qu et al., 2019)

located_in cell body

inferred from direct assay

(Qu et al., 2019)

located_in cell cortex

inferred from direct assay

(Qu et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in Golgi membrane

inferred from sequence or structural similarity with UniProtKB:Q06836

(Gene Ontology Curators, 2002-)

is_active_in ruffle membrane

inferred from biological aspect of ancestor with PANTHER:PTN002240921

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ARF GUANINE NUCLEOTIDE EXCHANGE FACTORS

Protein Family (UniProt)

Belongs to the PSD family. (E1JIT7)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ARF GUANINE NUCLEOTIDE EXCHANGE FACTORS -: ArfGEFs are Guanine Nucleotide Exchange Factors (GEFs) for ARF family GTPases. They are characterized by a central Sec7 catalytic domain domain with homology to yeast SEC7. (Adapted from PMID:15731001, FBrf0158741 and FBrf0220529).

Protein Function (UniProtKB)

Guanine nucleotide exchange factor for Arf6 (PubMed:28607459). Regulates axon growth and branching by inhibiting microtubule polymerisation at the cortex (PubMed:31718774). Together with shot, promotes axonal microtubule bundle integrity (PubMed:31718774). Required for normal ethanol-induced tolerance and preference (PubMed:28607459). Probably by activating Arf6, counteracts ethanol-induced sedation (PubMed:28607459).

(UniProt, E1JIT7)

Summary (Interactive Fly)

Guanine Nucleotide Exchange Factor (GEF) that directly inhibits microtubule growth - a cortical collapse factor that regulates to axon growth - sustains activation of Arf family GTPases - active in dynamic actin rearrangement during cell shape changes as well as cell patterning within epithelia

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Efa6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry E1JIT7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Efa6-RC

FBtr0113395

NM_001104403

5860

1387

Efa6-RD

FBtr0113396

NM_001104404

6262

1521

Efa6-RG

FBtr0302187

NM_170027

6332

1402

Efa6-RH

FBtr0334559

NM_001170220

6505

1601

Efa6-RI

FBtr0334560

NM_176541

7641

1554

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Efa6-PC

FBpp0112307

155.2

1387

6.30

FBpp0112308

168.2

1521

6.18

FBpp0291397

157.0

1402

6.25

FBpp0306626

176.4

1601

6.14

FBpp0306627

171.8

1554

6.09

E2QD56

NP_788718

AAO41590

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts (via MTED motif) with tubulin.

(UniProt, E1JIT7)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

E1JIT7

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Efa6 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Efa6_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.75

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

colocalizes_with adherens junction

inferred from direct assay

(Johnson et al., 2011)

located_in axon

inferred from direct assay

(Qu et al., 2019)

located_in cell body

inferred from direct assay

(Qu et al., 2019)

located_in cell cortex

inferred from direct assay

(Qu et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Efa6 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Efa6

View exonic expression by tissue for Dmel\Efa6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42665/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051158

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051158

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051158

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31158

Flygut - An atlas of the Drosophila adult midgut

FBgn0051158

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 16 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Efa6

Transgenic constructs containing regulatory region of Efa6

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6273

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Efa6^GD14945

viable, with Scer\GAL4^Mef2.PR

Efa6^GD14945

viable, with Scer\GAL4^pnr-MD237

Efa6^GD14945

viable, with Scer\GAL4^tin.CΔ4

Efa6^GD14945

Sterility

Allele

fertile

Efa6^c00953

Other Phenotypes

Allele

abnormal feeding behavior | adult stage | conditional (with Efa6^c00953)

Efa6^KO1

abnormal feeding behavior | adult stage | conditional (with Efa6^KO1)

Efa6^c00953

abnormal neuroanatomy | adult stage, with Scer\GAL4^ato.3.6

abnormal neuroanatomy | embryonic stage

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^ato.3.6

Efa6^UAS.EGFP

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PLu

abnormal neuroanatomy | P-stage, with Scer\GAL4^ppk.PU

abnormal neuroanatomy | P-stage (with Efa6¹²)

Efa6^GX6w-

abnormal neuroanatomy | P-stage (with Efa6^GX6w-)

Efa6¹²

abnormal neuroanatomy | P-stage | RU486 conditional, with Scer\GAL4^GSG2295

Efa6^VSH330083

abnormal neuroanatomy | P-stage | somatic clone

Efa6¹²

abnormal size | embryonic stage

abnormal size | embryonic stage, with Scer\GAL4^elav.PLu

chemical sensitive | adult stage (with Efa6^c00953)

Efa6^KO1

chemical sensitive | adult stage (with Efa6^KO1)

Efa6^c00953

chemical sensitive | adult stage | recessive

Efa6^c00953

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Efa6^GD14945

Phenotype manifest in

Allele

abdominal tergite 5 | female

Efa6^f03479

abdominal tergite 5 | female, with Scer\GAL4^Tub.PU

Efa6^GD14945

abdominal tergite 6 | female

Efa6^f03479

abdominal tergite 6 | female, with Scer\GAL4^pnr-MD237

Efa6^GD14945

abdominal tergite 6 | female, with Scer\GAL4^Tub.PU

Efa6^GD14945

axon | adult stage, with Scer\GAL4^GMR31F10

Efa6^GD14945

axon | embryonic stage

axon | embryonic stage, with Scer\GAL4^elav.PLu

axonal growth cone | embryonic stage

axon collateral | adult stage, with Scer\GAL4^ato.3.6

Efa6^UAS.EGFP

axon collateral | adult stage | increased number

Efa6^KO1

axon collateral | adult stage | increased number, with Scer\GAL4^ato.3.6

Efa6^GD14945

axon collateral | embryonic stage, with Scer\GAL4^ato.3.6

Efa6^UAS.EGFP

axon collateral | embryonic stage | increased number

Efa6^KO1

axon microtubule bundle | adult stage, with Scer\GAL4^GMR31F10

Efa6^GD14945

axon microtubule bundle | embryonic stage

Efa6^GX6w+

axon microtubule bundle | embryonic stage, with Scer\GAL4^elav.PLu

Efa6^GD14945

dendrite | P-stage, with Scer\GAL4^ppk.PU

dendrite | P-stage (with Efa6¹²)

Efa6^GX6w-

dendrite | P-stage (with Efa6^GX6w-)

Efa6¹²

dendrite | P-stage | RU486 conditional, with Scer\GAL4^GSG2295

Efa6^VSH330083

dendrite | P-stage | somatic clone

Efa6¹²

embryonic/larval sensory neuron | larval stage, with Scer\GAL4⁴⁷⁷

embryonic/larval sensory neuron | larval stage, with Scer\GAL4^ppk.PU

embryonic/larval sensory neuron | larval stage (with Efa6¹²)

Efa6^GX6w-

embryonic/larval sensory neuron | larval stage (with Efa6^GX6w-)

Efa6¹²

embryonic/larval sensory neuron | P-stage, with Scer\GAL4^ppk.PU

embryonic/larval sensory neuron | P-stage (with Efa6¹²)

Efa6^GX6w-

embryonic/larval sensory neuron | P-stage (with Efa6^GX6w-)

Efa6¹²

embryonic/larval sensory neuron | P-stage | RU486 conditional, with Scer\GAL4^GSG2295

Efa6^VSH330083

embryonic/larval sensory neuron | P-stage | somatic clone

Efa6¹²

filopodium | embryonic stage

Efa6^KO1

filopodium | embryonic stage, with Scer\GAL4^elav.PLu

Efa6^UAS.EGFP

growth cone | embryonic stage, with Scer\GAL4^elav.PLu

Efa6^UAS.EGFP

growth cone filopodium | embryonic stage

Efa6^GX6w-

lobula columnar neuron LC14 | adult stage, with Scer\GAL4^ato.3.6

medulla | adult stage, with Scer\GAL4^ato.3.6

medulla | adult stage, with Scer\GAL4^GMR31F10

Efa6^GD14945

microtubule | embryonic stage, with Scer\GAL4^elav.PLu

Efa6^UAS.EGFP

microtubule | embryonic stage | progressive

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (49)

Hsap\PSD

10 of 14

Yes

Hsap\PSD2

8 of 14

Yes

Hsap\PSD3

8 of 14

Yes

Hsap\PSD4

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\PLEKHA2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\SKAP2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (44)

Rnor\Psd

10 of 14

Yes

Rnor\Psd2

8 of 14

Yes

Rnor\Psd3

7 of 14

Yes

Rnor\Psd4

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Plekha2

1 of 14

Yes

Rnor\Sh3yl1

1 of 14

Yes

Rnor\Skap1

1 of 14

Yes

Rnor\Skap2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (42)

Mmus\Psd

10 of 14

Yes

Mmus\Psd2

8 of 14

Yes

Mmus\Psd3

8 of 14

Yes

Mmus\Psd4

5 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Plekha2

1 of 14

Yes

Mmus\Skap1

1 of 14

Yes

Mmus\Skap2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (26)

Xtro\psd4

5 of 13

Yes

Xtro\psd

5 of 13

Yes

Xtro\psd3

3 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (69)

Drer\psda

9 of 14

Yes

Drer\psd2

7 of 14

Yes

Drer\LOC101886286

6 of 14

Yes

Drer\psd3l

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\plekha1a

1 of 14

Yes

Drer\plekha1b

1 of 14

Yes

Drer\plekha2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:dkey-33c12.10

1 of 14

Drer\si:dkey-33c12.12

1 of 14

Drer\si:dkey-33c12.13

1 of 14

Drer\si:dkey-84o3.2

1 of 14

Drer\si:dkey-112g5.13

1 of 14

Drer\si:dkey-112g5.16

1 of 14

Drer\si:dkey-276j7.2

1 of 14

Drer\si:zfos-1837d3.1

1 of 14

Drer\skap1

1 of 14

Yes

Drer\skap2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\efa-6

9 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (15)

Agam\AgaP_AGAP000774

8 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (10)

Atha\GN

3 of 13

Yes

Atha\GNL1

3 of 13

Yes

Atha\GNL2

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\GEA1

4 of 13

Yes

Scer\GEA2

4 of 13

Yes

Scer\SYT1

3 of 13

Yes

Scer\SEC7

2 of 13

Scer\YEL1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\sec72

2 of 12

Yes

Spom\sec73

2 of 12

Yes

Spom\sec74

2 of 12

Yes

Spom\SPAC11E3.11c

2 of 12

Yes

Spom\SPBC211.03c

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Efa6. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (13)

4 of 13

4 of 13

4 of 13

4 of 13

2 of 13

2 of 13

1 of 13

bbg

1 of 13

β-Spec

1 of 13

kst

1 of 13

loco

1 of 13

sif

1 of 13

ssp6

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

alcohol, response to, PSD-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Efa6^c00953

model of alcohol use disorder

CEA

(Gonzalez et al., 2018)

CEA with Efa6^KO1

(Gonzalez et al., 2018)

Efa6^KO1

model of alcohol use disorder

CEA with Efa6^c00953

(Gonzalez et al., 2018)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Efa6^c00953

ameliorates alcohol use disorder

modeled by RhoGAP18B¹

(Gonzalez et al., 2018)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PSD; pleckstrin and Sec7 domain containing

10 of 14

602327

PSD2; pleckstrin and Sec7 domain containing 2

8 of 14

620656

PSD3; pleckstrin and Sec7 domain containing 3

8 of 14

614440

PSD4; pleckstrin and Sec7 domain containing 4

6 of 14

614442

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Qu et al., 2019)

enhanceable

(Bu et al., 2021)

suppressible

(Bu et al., 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cindr

enhanceable

Efa6

(Johnson et al., 2011)

Dcr-2|Arf6

enhanceable

Efa6

(Johnson et al., 2011)

RhoGAP18B

suppressible

Efa6

(Gonzalez et al., 2018)

enhanceable

(Qu et al., 2019)

suppressible

(Qu et al., 2019)

enhanceable

(Bu et al., 2021)

External Data

Subunit Structure (UniProtKB)

Interacts (via MTED motif) with tubulin.

(UniProt, E1JIT7 )

Linkouts

BioGRID - A database of protein and genetic interactions.

67619

DroID - A comprehensive database of gene and protein interactions.

FBgn0051158

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Endocytosis

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A8JR81

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-75

Cytogenetic map

94B5-94B6

Sequence location

3R:22,587,755..22,608,842 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

94B5-94B6

Limits computationally determined from genome sequence between P{lacW}Dph5^L4910 and P{lacW}Gclm^L0580

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-75

(FlyBase, 2016)

3-77.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (17)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

60588

w^*; TI{TI}Efa6^GFP-C

10314

w¹¹¹⁸; PBac{PB}Efa6^c00953

93285

w¹¹¹⁸; PBac{UAS-Efa6.FL.EGFP}VK00031

604993

y¹ v¹; P{TOE.GS02804}attP40

93286

w¹¹¹⁸; PBac{UAS-Efa6.Nterm.EGFP}VK00031

44321

y¹ w^*; Mi{MIC}Efa6^MI07107

92528

y¹ v¹; P{U6.2-Efa6.dgRNA}attP40/CyO

60587

w^*; TI{TI}Efa6^GX6w-/TM6B, Tb¹

99666

y¹ w^*; PBac{UAS-Efa6.ΔCterm.EGFP}VK00031

99665

y¹ w^*; PBac{UAS-Efa6.Nterm.CAAX.EGFP}VK00031

VDRC

v330083

P{VSH330083}attP40

v42321

w¹¹¹⁸; P{GD14945}v42321

More stocks available...

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (49)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD20094

Other clones

MIP03119

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0051158

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0051158

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Bu et al., 2021)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG18185 CG6941

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG18185 and CG6941 merged as CG31158 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Efa6 CG31158

(Johnson et al., 2011)

Nomenclature comments

Etymology

Named "Efa6" after the human ortholog.

(FlyBase, 2009-)

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG18185

CG31158

(Trivedi et al., 2020, Zeledon et al., 2019, Meadows, 2017.1.11, Balakrishnan et al., 2015, Chang et al., 2015, Ashwal-Fluss et al., 2014, Terriente-Felix et al., 2013, Johnson et al., 2011, Bina et al., 2010, Ni et al., 2010.12.1, Huang et al., 2009, Guo et al., 2008)

CG6941

(Casanova, 2007)

EFA6

(Cox et al., 2004)

Efa6

(Chvilicek et al., 2024, Liu et al., 2024, Peterson and Ahmad, 2024, Liu et al., 2023, Dzaki et al., 2022, Bu et al., 2021, Lathen et al., 2020, Qu et al., 2019, Yee et al., 2019, Zeledon et al., 2019, Gonzalez et al., 2018, Lowenstein and Velazquez-Ulloa, 2018, Podolsky et al., 2018, Transgenic RNAi Project members, 2017-, Dembeck et al., 2015, Gene Disruption Project members, 2015-)

dEfa6

(Johnson et al., 2011)

dPsd

(Johnson et al., 2011)

efa6

(Bu et al., 2022, Kamiyama et al., 2021)

Name Synonyms

Efa6 pleckstrin and Sec7 domain containing

Exchange factor for Arf 6

Exchange factor for Arf 6 ortholog

Secondary FlyBase IDs

FBgn0038990
FBgn0038991

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42665

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

E1JIT7

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

E1JIT7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

E1JIT7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42665/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051158

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051158

FlyMine - An integrated database for Drosophila genomics

FBgn0051158

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31158

MARRVEL_MODEL - MARRVEL (model organism gene)

42665

Linkouts

BioGRID - A database of protein and genetic interactions.

67619

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0051158

DroID - A comprehensive database of gene and protein interactions.

FBgn0051158

DRSC - Results frm RNAi screens

FBgn0051158

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Efa6_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051158

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0051158

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31158

Flygut - An atlas of the Drosophila adult midgut

FBgn0051158

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011095

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Endocytosis

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A8JR81

References (62)

The following external sites may use different assemblies or annotations than FlyBase.

-0.75

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