FlyBase Allele Report: Dmel\Efa6[KO1]

General Information

Symbol

Dmel\Efa6^KO1

Species

D. melanogaster

Name

FlyBase ID

FBal0247919

Feature type

allele

Associated gene

Dmel\Efa6

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Huang et al., 2009)

ends-out gene targeting

(Huang et al., 2009)

Progenitor genotype

Cytology

Description

74bp deletion in exon 8.

(Huang et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA with Efa6^c00953

(Gonzalez et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal feeding behavior | adult stage | conditional (with Efa6^c00953)

(Gonzalez et al., 2018)

abnormal neuroanatomy | embryonic stage

(Qu et al., 2019)

abnormal size | embryonic stage

(Qu et al., 2019)

chemical sensitive | adult stage (with Efa6^c00953)

(Gonzalez et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Cultured neurons from stage 11 Efa6^KO1 embryos show a small but significant increase in axon length, with a significantly higher density of branches, as compared to controls. Growth cones show increased microtubule polymerization, as there is a significant increase in the number of EB1 comets at growth cones and growth cone filopodia. There are significantly more growing microtubules (EB1 comets) in filopodia along the axon shaft, but not in the axon shaft itself, as compared to controls. Axonal microtubules become progressively more disorganized, as compared to controls.

(Qu et al., 2019)

Efa6^c00953/Efa6^KO1 are more sensitive (lower survival) to ethanol than wild-type controls.

Naive Efa6^c00953/Efa6^KO1 mutants show preference for ethanol-laced food, unlike the aversion normally observed in wild-type controls. Efa6^c00953/Efa6^KO1 mutants previously exposed to ethanol show the same preference for ethanol-laced food as wild-type controls.

(Gonzalez et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Efa6^KO1/CG31158[+] is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2011)

Efa6^KO1/CG31158[+] is an enhancer of retina phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Efa6^KO1/CG31158[+] is an enhancer of ommatidium phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Efa6^KO1/CG31158[+] is an enhancer of primary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Efa6^KO1/CG31158[+] is an enhancer of secondary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Efa6^KO1/CG31158[+] is an enhancer of tertiary pigment cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Efa6^KO1/CG31158[+] is an enhancer of cone cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Efa6^KO1/CG31158[+] is an enhancer of interommatidial precursor cell phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The eye mis-patterning phenotype resulting from the co-expression of Arf51F^GD13822 with Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF is significantly enhanced in Efa6^KO1 heterozygotes. The number of correctly patterned secondary and tertiary cell is reduced. The total number of interommatidial precursor cells (IPCs) does not differ markedly from the wild-type number of 12, indicating that the enhancement is due to specifically to increased disorder in IPC patterning. Additionally, errors in the placement of three bristle groups about each ommatidium and errors in primary pigment cell and cone cell patterning and ommatidial rotation are modestly enhanced.

The ommatidial patterning errors resulting from the expression of cindr^{dsRNA.PC.PD.Scer\UAS} via Scer\GAL4^GMR.PF are strongly enhanced in Efa6^KO1 heterozygotes.

(Johnson et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93284

w¹¹¹⁸; Efa6^KO1/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG31158^KO#1

(Huang et al., 2009)

CG31158^KO1

Efa6^KO#1

(Qu et al., 2019)

Efa6^KO1

Efa6^KO

(Gonzalez et al., 2018)

dPsd^KO#1

(Johnson et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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