FlyBase Allele Report: Dmel\Efa6[GX6w-]

General Information

Symbol

Dmel\Efa6^GX6w-

Species

D. melanogaster

Name

FlyBase ID

FBal0247920

Feature type

allele

Associated gene

Dmel\Efa6

Associated Insertion(s)

TI{TI}Efa6^GX6w-

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Huang et al., 2009)

Progenitor genotype

Associated Insertion(s)

TI{TI}Efa6^GX6w-

Cytology

Description

Most of the Efa6 coding region has been deleted. A 50bp version of the attP site and a loxP site are present in place of the deleted sequence.

(Huang et al., 2009)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:22,598,356..22,605,777 [+]

Comment:

Most of the Efa6 coding region has been deleted. A 50bp version of the attP site and a loxP cassette containing a w^+* marker are present in place of the deleted sequence. The deletion was reported to map to 3R:18 ,424,078..18,431,499 in release 5 coordinates.

(Huang et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Qu et al., 2019)

abnormal neuroanatomy | P-stage (with Efa6¹²)

(Bu et al., 2021)

abnormal size | embryonic stage

(Qu et al., 2019)

Phenotype Manifest In

axon | embryonic stage

(Qu et al., 2019)

axonal growth cone | embryonic stage

(Qu et al., 2019)

dendrite | P-stage (with Efa6¹²)

(Bu et al., 2021)

embryonic/larval sensory neuron | larval stage (with Efa6¹²)

(Bu et al., 2021)

embryonic/larval sensory neuron | P-stage (with Efa6¹²)

(Bu et al., 2021)

growth cone filopodium | embryonic stage

(Qu et al., 2019)

microtubule | embryonic stage | progressive

(Qu et al., 2019)

Detailed Description

Statement

Reference

Cultured neurons from stage 11 Efa6^GX6w- embryos show a small but significant increase in axon length, as compared to controls. Growth cones show increased microtubule polymerization, as there is a significant increase in the number of EB1 comets at growth cones and growth cone filopodia; although comet velocity is unaffected, their lifetime is significantly longer, particularly at the tip of filopodia, as compared to controls; more microtubules are found at the filopodia than in controls. The axon shalt shows no major changes in growing microtubule dynamics (EB1 comet number, lifetime, velocity or directionality), but more filopodia along the axon shaft contain microtubules. Axonal microtubules are significantly more disorganized than in controls.

(Qu et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Efa6^GX6w-/Efa6¹² has abnormal neuroanatomy | P-stage phenotype, enhanceable by Scer\GAL4^ppk.PU/stai^GL01099

(Bu et al., 2021)

NOT Enhanced by