Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

Source was live S2R+ cells; proteins produced from transfected constructs.

Bait was fused to a mitochondial anchoring tag. Interaction was determined by colocalization.

Bait was fused to a pleckstrin domain with a tag. It removed nuclear proteins to the cytoplasm. Interaction was detected as movement from nuclear proteins to the cytoplasm.

Source was live S2R+ cells; proteins produced from transfected constructs.