FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
me31B, Gyf
FlyBase ID
FBig0000119970
Interaction Network
Interactions Browser links
me31B network
Gyf network
Reported Interactions
FBrf0243046-2.coIP.WB
Reference
Ruscica et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Gyf 

GIGYF

bait

GFP tag

Gyf 
Gyf 
me31B 

Me31B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyf 
N-terminal domain
sufficient binding region
aa 1-640

coordinates relative to Gyf-PG

Gyf 
me31B-binding domain
necessary binding region
aa 331-374

coordinates relative to Gyf-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Ruscica et al., 2019)
FBrf0244109-1.coIP.WB
Reference
Peter et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Gyf 

GIGYF

bait

HA tag

Gyf 
Gyf 
me31B 

Me31B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyf 
MBM domain
sufficient binding region
aa 341-369

coordinates relative to Gyf-PG

Gyf 
MBM domain
necessary binding region
aa 341-369

coordinates relative to Gyf-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Peter et al., 2019)
FBrf0244109-2.coIP.WB
Reference
Peter et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

bait

GFP tag

me31B 
me31B 
me31B 
Gyf 

GIGYF

prey

HA tag

Gyf 
Gyf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
me31B 
RecA2 domain
sufficient binding region
aa 268-459

coordinates relative to me31B-PA

me31B 
FDF pocket residues 285,289
mutation decreasing interaction
aa 285,289

C285A,L289A, coordinates relative to me31B-PA

me31B 
W pocket residues 310,314
mutation decreasing interaction
aa 310,314

L310A,K314A, coordinates relative to me31B-PA

Gyf 
MBM domain residue 349
mutation disrupting interaction
aa 349

W349A, coordinates relative to Gyf-PG

Gyf 
MBM domain residues 361,367
mutation disrupting interaction
aa 361,367

F361A,F367A, coordinates relative to Gyf-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Peter et al., 2019)
FBrf0244109-3.PD.MW
Reference
Peter et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

bait

GST tag

me31B 
Gyf 

GIGYF

prey

MBP tag

Gyf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gyf 
MBM domain
sufficient binding region
aa 341-369

coordinates relative to Gyf-PG

me31B 
RecA2 domain
sufficient binding region
aa 264-431

coordinates relative to me31B-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Peter et al., 2019)
FBrf0244109-4.XC
Reference
Peter et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

neutral component
me31B 

Me31B

neutral component
me31B 
me31B 

Me31B

neutral component
me31B 
Gyf 

GIGYF

neutral component
Gyf 
Gyf 

GIGYF

neutral component
Gyf 

GIGYF

neutral component
Gyf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
me31B 
RecA2 domain
sufficient binding region
aa 264-431

coordinates relative to me31B-PA

Gyf 
MBM domain
sufficient binding region
aa 342-368

coordinates relative to Gyf-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; me31B protein produced as recombinant fusion protein in bacterial system; Gyf peptide synthesized commercially.

(Peter et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (2)