FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rheb, CCT4
FlyBase ID
FBig0000120002
Interaction Network
Interactions Browser links
Rheb network
CCT4 network
Reported Interactions
FBrf0242663-1.coIP.WB
Reference
Kim and Choi, 2019
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CCT4 

CCT4

bait
Rheb 

Rheb

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kim and Choi, 2019)
FBrf0242663-3.PD.WB
Reference
Kim and Choi, 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rheb 

Rheb

bait

MBP tag

Rheb 
CCT4 

CCT4

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rheb 
interaction domain
mutation decreasing interaction
aa 49-63

deletion

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Kim and Choi, 2019)
FBrf0242663-4.PD.WB
Reference
Kim and Choi, 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CCT4 

CCT4

bait

GST tag

Rheb 

Rheb

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Kim and Choi, 2019)
FBrf0242663-9.coIP.WB
Reference
Kim and Choi, 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rheb 

Rheb

bait

V5 tag

CCT4 

CCT4

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kim and Choi, 2019)
External Crossreferences and Linkouts ( 0 )
References (1)