FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
PI31, ctp
FlyBase ID
FBig0000120075
Interaction Network
Interactions Browser links
PI31 network
ctp network
Reported Interactions
FBrf0243279-1.coIP.MS
Reference
Liu et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PI31 

PI31

bait

HA tag

PI31 
ctp 

dDYNLL1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PI31 
phosphorylation site
mutation decreasing interaction
aa 168

S168A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Liu et al., 2019)
FBrf0243279-10.PD.WB
Reference
Liu et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PI31 

PI31

bait

GST tag

PI31 
ctp 

dDYNLL1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PI31 
phosphorylation site
mutation increasing interaction
aa 168

S168D or S168E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Liu et al., 2019)
FBrf0243279-11.coIP.MS
Reference
Liu et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ctp 

dDYNLL1

bait

FLAG tag

PI31 

PI31

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Liu et al., 2019)
FBrf0243279-14.PD.WB
Reference
Liu et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ctp 

dDYNLL1

bait

FLAG tag

PI31 

PI31

prey

GST tag

PI31 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PI31 
phosphorylation site
mutation increasing interaction
aa 168

S168D or S168E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Liu et al., 2019)
FBrf0243279-4.coIP.WB
Reference
Liu et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PI31 

PI31

bait

HA tag

PI31 
ctp 

dDYNLL1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PI31 
phosphorylation site
mutation decreasing interaction
aa 168

S168A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Liu et al., 2019)
FBrf0243279-7.coIP.WB
Reference
Liu et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ctp 

dDYNLL1

bait

FLAG tag

PI31 

PI31

prey

HA tag

PI31 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PI31 
phosphorylation site
mutation decreasing interaction
aa 168

S168A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct or endogenous gene.

(Liu et al., 2019)
FBrf0243279-8.coIP.WB
Reference
Liu et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
PI31 

PI31

bait

HA tag

PI31 
ctp 

dDYNLL1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PI31 
phosphorylation site
mutation decreasing interaction
aa 168

S168A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Liu et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)