The molecular weight determined by analytical ultracentrifugation is consistent with a spas hexamer. A stabilizing E583Q mutant form of spas that prevents ATP hydrolysis was used.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Cryo-EM of spas reveals a hexameric structure. A stabilizing E583Q mutant form of spas that prevents ATP hydrolysis was used. A polyglutamate peptide was also added to improve hexamer stability.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.