FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tup, pnr
FlyBase ID
FBig0000120184
Interaction Network
Interactions Browser links
tup network
pnr network
Reported Interactions
FBrf0190092-5.coIP.WB
Reference
Biryukova and Heitzler, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tup 

Isl

bait

FLAG tag

pnr 

Pnr

prey

Myc tag

pnr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnr 
Zinc fingers 1 and 2
necessary binding region
aa 158-250
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Biryukova and Heitzler, 2005)
FBrf0190092-6.PD.A
Reference
Biryukova and Heitzler, 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tup 

Isl

bait

GST tag

tup 
pnr 

Pnr

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tup 
LIM domain
sufficient binding region
aa 53-171
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Biryukova and Heitzler, 2005)
External Crossreferences and Linkouts ( 0 )
References (1)