FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000120215
Interaction Network
Interactions Browser links
Cenp-C network
Reported Interactions
FBrf0243509-1.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CENP-C

neutral component

CENP-C

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cupin domain
sufficient binding region
aa 1190-1411
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cenp-C is a dimer in crystallo.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

FBrf0243509-2.CS.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CENP-C

neutral component

CENP-C

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cupin domain
sufficient binding region
aa 1190-1411
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Cenp-C is a dimer in solution.

FBrf0243708-10.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dCENP-C

neutral component

dCENP-C

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
self-association domain
sufficient binding region
aa 1264-1411
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

The molecular weight of Cenp-C estimated by SEC-MALS is consistent with a dimer.

FBrf0243708-9.PBD.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dCENP-C

bait

GFP tag

dCENP-C

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
self-association domain
sufficient binding region
aa 1264-1411
self-association domain residues 1357 and 1406
mutation disrupting interaction
aa 1357,1406

L1357E,L1406E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was U2OS cells; bait produced from transfected construct; prey produced from transfected construct.

The ability of Cenp-C fused to LacI to recruit HA-tagged Cenp-C to LacO arrays was taken as evidence of Cenp-C self-association.

FBrf0245264-14.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CENP-C

neutral component

His tag

CENP-C

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal domain
sufficient binding region
aa 1264-1411
C-terminal domain residues 1357,1407
mutation disrupting interaction
aa 1357,1407

L1357E,M1407E, coordinates relative to Cenp-C-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

The measured MW of Cenp-C is consistent with a dimer.

FBrf0245264-18.CS.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CENP-C

neutral component

CENP-C

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal domain
sufficient binding region
aa 1264-1411
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Cosedimentation analysis is consistent with Cenp-C forming a dimer.

External Crossreferences and Linkouts ( 0 )
References (3)